| Project/Area Number |
62540544
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
動物発生・生理学
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| Research Institution | Nagoya University |
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Principal Investigator |
NAKANISHI Yasuo Nagoya Univ., Faculty of Science, Department of Chemistry, 理学部, 助教授 (40022636)
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| Project Period (FY) |
1987 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1988: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1987: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | Salivary gland / Morphogenesis / Epithelial-mesenchymal interaction / Cleft formation / Collagen / Heparitinase / Heparan sulfate / 三次元表示 / 上皮-間充識相互作用 / 上皮-問充織相互作用 / コラゲナーゼ / X線照射 / プロテオヘパラン硫酸 |
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| Research Abstract |
The purpose of this study was to demonstrate the involvement of extracellular matrix components and also to clarify the relationship between the protruding activity of the epithelium and the shapechanging force of the mesenchyme during morphogenesis of mouse embryonic submandibular gland. The findings are summarized as summarized as follows:
(1) The collagen type necessary for cleft initiation was found to be collagen III, based on the Facts that an interstitial collagenase from bovine dental pulp inhibited the branching and immunohistochemical staining demonstrated the accumulation of collagen III at the cleft points.
(2) The three-dimensional computer reconstruction made from serial sections stained with the anti-collagen III antibody revealed that collagen III encircles the lobule alond the cleft so as to separate it into smaller ones.
(3) The highly purified preparation of bacterial collagenase inhibited the bead-separation activity of the mesenchyme, indicating that the flowing movement of the cells in the tissue is under the influence of collagen.
(4) The heparitinase free of other proteins halted the epithelial branching and growth. Also, submandibular glands cultured in the presence of the enzyme did not contain heparan sulfate in the tissues, suggesting that heparan sultate proteoglycans are involved in the morphogenesis.
(5) Unfortunately, we have been unsuccessful in finding a epithelial growth factor which may be useful for elucidating the relationship between the expanding activity of the epithelium and the shape-changing activity of the mesenchyme.
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