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X-ray microanalytical and cytochemical studies on the calcium accumulation machanism.

Research Project

Project/Area Number 62540570
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 動物形態・分類学
Research InstitutionYamaguchi University

Principal Investigator

YAMAOKA Ikuo  Yamaguchi University, Professor, 理学部, 教授 (30034705)

Co-Investigator(Kenkyū-buntansha) MURAKAMI Ryutaro  Yamaguchi University, Assistant, 理学部, 助手 (40182109)
Project Period (FY) 1987 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1988: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1987: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsCalcium accumulation / Alkaline phosphatase / Amoeba / Fibrocartilage / Os penis / Testosterone / X-ray microanalysis / 器官培養 / カルシウム沈着 / 有殻アメーバ / Golgi apparatus / 表面被覆 / 分析電子顕微鏡
Research Abstract

Study on the calcification in the cell and tissue of animal organism has an important problem relating to the occification in the bone and dental formation.
In our project in order to understand the calcium accumulation mechanism two model systems were examined: one was a special granule containing calcium in an amoeba (Cochliopodium sp.), the other was the calcification in the fibrocartilage of the os penis of the rat.
In the amoeba the special granules were situated near the nucleus and were able to control the appearance rate. When the amoeba transplanted in the agar containing EGTA the special granule disappeared at hours 12. When the amoeba returened to the normal condition the granules reappeared, but not appeared in the agar containing levamisol which was a specific inhibitor to alkaline phosphatase activity (ALPase).
In the embrionec fibrocartilage cultured in vitro ALPase activities which depend on testosterone were detected at 14 days after culture. The calcium deposition occured in the matrix at 21 days.
These results suggest that the calcium accumulation mechabism relates to exsist of the alkaline phosphatase activity.

Report

(3 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Yamaoka,I.: Zool.Sci.4. 985 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Murakami,R.: Zool.Sci.4. 1055 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Miyake,K.: Proc.Chugoku-Shikoku Branch Zool.Soc.Jap.40. 20 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yamaoka, I.: "Regulation of the intracellular calcium storage in an amoeba, Cochliopodium sp.." Zool. Sci.4. 995 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Murakami, R.: "Development of collagenous matrix in the fibrocartilage of the os penis of the rat." Zool. Sci.4. 1055 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Miyake, K.: "Effects of testosterone on the fibrocartilage of the os penis cultured in vitro." Proc. Chugoku-Shikoku Branch Zool. Soc. Jap.40. 20 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Murakami, R.: "Testosterone dependent characterization on the fibrocartilage of the os penis cultured in vitro."

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yamaoka, I.: "X-ray microanalysis and histochemistry of a special granule in an amoeba, Cochliopodium sp."

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yamaoka,I.: Zool.Sci.4. 995 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] Murakami,R.: Zool.Sci.4. 1055 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] Miyake,K.: Proc.Chugoku-shikoku Branch Zool.Soc.Jap.40. 20 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] I. Yamaoka: Zoological Science. 4. 995 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] I. Yamaoka: In prparation.

    • Related Report
      1987 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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