| Project/Area Number |
62560010
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Breeding science
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| Research Institution | National Institute of Genetics |
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Principal Investigator |
SANO Yoshio Natl.Inst.Genetics, Dept. Integrated Genetics, Associate Professor, 総合遺伝研究系, 助教授 (70109528)
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| Project Period (FY) |
1987 – 1989
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| Project Status |
Completed (Fiscal Year 1989)
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| Budget Amount *help |
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1989: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1988: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1987: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | Gene expression / waxy gene / Rice / Amylose content / Multiple alleles / アミロース含量 / アミロース |
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| Research Abstract |
The waxy (wx) locus in rice, which specifies a major starchgranule-bound protein called Wx protein, is genetically well characterized. Our previous works revealed that in addition to wx, there are at least two nonwaxy alleles at the wx locus which regulate the amounts of the gene product as well as amylose in different ways. The present study was carried out to get more insights on to what extent the allelic differentiation at the wx locus is important for diversification in amylose content observed among nonwaxy rice cultivars. The results confirmed that among naturally occurring variants tested, amylose content is mainly determined by the allelic changes at the wx locus. In addition, the amount of the gene product was affected by effects of temperatures, modifiers and the gene dosage in a similar manner which the amylose content was affected. Those effects were expected to lead the amylose content to a polygenic trait in segregating populations as often reported in rice, even though the amylose content was simply controlled by the amount of Wx protein. The allelic differentiation at the wx locus was also discussed in relation to the evolutionary significances of the gene regulation.
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