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Studies on Metabolizing Activities toward Nitrogen- and Sugar-Compounds in Bifidobacteria and Their Application

Research Project

Project/Area Number 62560101
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 発酵・醸造
Research InstitutionKyoto University

Principal Investigator

YANO Toshihiro (1988)  Research Officer, Faculty of Agriculture, Kyoto University, 農学部, 教務職員 (30135553)

立木 隆 (1987)  京都大学, 農学部, 助手 (60026573)

Co-Investigator(Kenkyū-buntansha) TACHIKI Takashi  Associate Professor, Faculty of Science and Engineering, Ritsumeikan University, 理工学部, 助教授 (60026573)
矢野 俊博  京都大学, 農学部, 教務職員 (30135553)
Project Period (FY) 1987 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1988: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 1987: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsBifidobacteria / Enzymatic synthesis of oligosaccharides assimilable by bifidobacteria / Glutamate dehydrogenase / Glutamine synthetase / -D-Fucosylmannitol / -D-Glucosidase I / 特異的資化糖の酵素合成 / βーDグルコシダーゼI / アンモニア代謝 / 糖質代謝
Research Abstract

1. Bifidobacterial enzymes participating in ammonia metabolism.
(1) Inactivation of glutamine synthetase (GS) in bifidobacteria was caused by enzymatic adenylylation of GS protein, and the adenylylation enzyme of bifidobacterium pseudolongum was characterized. The changes in the properties of the GS-reactions after the action of the enzyme were different from the findings obtained with enterobacteria but similar to those in a few species of actinomycetes.
(2) Glutamate dehydrogenases (GDHs) were purified from some bifidobacteria. Their enzymatic properties indicated that the enzymes catalyze reductive amination pysiologically rather than oxidative deamination, and suggested that they play some important role in ammonia metabolism together with GS.
(3) Analyses of variation of ammonia concentration in feces of a breast-fed infant suggested that bifidobacterial cells and GS might be concerned with elimination of ammonia in the lower intestines.
2. Properties and utilization of oligosaccharid … More e-hydrolyzing enzymes of bifidobacteria.
(1) A simple method was established for prepartion of a new -D-glucosidase ( -D-glucosid-ase I) from bifidobacterium breve 203, which has desirable properties for practical use.
(2) -D-glucosidase I hydrolyzed not only p-nitrophenyl (p-NP) -D-glucoside but also p-BP -D-fucoside and, furtheremore, transferred -D-fucosyl moiety to other sugars or sugar derivatives. By use of the enzyme, -D-fucosylglucose was synthesized in the mixture containing glucose as an acceptor with a yield of 52 % based on -D-fucosyl donor (p-NP -D-fucoside). The product consisted of four isomers ( 1-2, 1-3, 1,4 and 1-6), and it suppoeted specifically bifidobacterial growth. -D-fucosylmannitol was synthesized similarly with a yield of 45 %. It contained at least two isomers, however, their chemical structures and assimilability by bifidobacteria were not revealed in this study.
Formation of new substance was confirmed in reaction mixture containing maltose, xylose, glycerol or sorbitol as an acceptor. Less

Report

(3 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] 酒井,謙二: Agricultural and Biological Chemistry. 53. 313-318 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 畑中,勝行: Agricultural and Biological Chemistry.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 立木,隆: Agricultural and Biological Chemistry.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 矢野,俊博: Agricultural and Biological Chemistry.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Sakai, Kenji: "Synthesis of -D-Fucosylglucose by Using -D-Glucosidase I of Bifidobacterium breve 203 and Its Assimilation by Bifidobacteria" Agricultural and Biological Chemistry. 53. 313-318 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Hatanaka, Masayuki: "Properties of Glutamate Dehydrogenases from Bifidobacteria" Agricultural and Biological Chemistry.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tachiki, Takashi: "Glutamine Synthetase-Adenylylating Enzyme from Bifidobacterium pseudolongum" Agricultural and Biological Chemistry.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yano, Toshihiro: "Glutamine Formation from Ammonia and Glutamate in Feces by Coupling of Bifidobacterial Cells and Glutamine Synthetase" Agricultural and Biological Chemistry.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 立木隆: Agricultural and Biological Chemistry.

    • Related Report
      1988 Annual Research Report
  • [Publications] 矢野俊博: Agricultural and Biological Chemistry.

    • Related Report
      1988 Annual Research Report
  • [Publications] 畑中勝行: Agricultural and Biological Chemistry. 52. (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] 酒井謙二: Agricultural and Biological Chemistry. 52. (1988)

    • Related Report
      1987 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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