Construction of host・vector system in mold: Function and structure of extranuclear DNA in fungi

Research Project

Project/Area Number	62560103
Research Category	Grant-in-Aid for General Scientific Research (C)
Allocation Type	Single-year Grants
Research Field	発酵・醸造
Research Institution	Kyoto University
Principal Investigator	MURATA Kousaku Research Institute for Food Science, Kyoto University, 食糧科学研究所, 助教授 (90142299)
Co-Investigator(Kenkyū-buntansha)	SAKAGUCHI Morihiko Research Institute for Food Science, Kyoto University, 食糧科学研究所, 助教授 (00027187)
Project Period (FY)	1987 – 1988
Project Status	Completed (Fiscal Year 1988)
Budget Amount *help	¥1,900,000 (Direct Cost: ¥1,900,000) Fiscal Year 1988: ¥300,000 (Direct Cost: ¥300,000) Fiscal Year 1987: ¥1,600,000 (Direct Cost: ¥1,600,000)
Keywords	Mold / Host・vector system / Plasmid DNA / 形質転換 / 細胞融合
Research Abstract	Fungi is industrially important microbe and various useful enzymes and organic acids are producing by using fungi cells in a large industrial scale. To breed more useful fungi, we intended to construct host-vector systems available in fungi. For the construction of the system, plasmid DNAs are required to clone and amplify the genes in fungi cells. However, we have no plasmid DNAs available in fungi system, and yeast plasmid DNAs have been utilized, although the yeast plasmid DNAs are unstable in fungi cells and frequently integrated into fungi genomes. To overcome this barrier, the use of plasmid DNAs inherent to fungi is thought to be suitable. So, we screened the plasmid DNAs in various fungi cells. despite the voluminous screening experiments, we could not find the plasmid DNAs, except that plasmid DNA-like molecule was found in Mucor jansence. On the other hand, we purified glyoxalase I and methylglyoxal reductases from Aspergillus niger. We are now attempting to construct host-vector systems by using plasmid DNA we found in M. ransence and genes for these enzymes.