PRODUCTION OF TETRAPYRROLE COENZYMES BY A METHANOGEN
| Project/Area Number |
62560109
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
発酵・醸造
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
NISHIO Naomichi FAC. OF ENGINEERING, HIROSHIMA UNIV., ASSOC. PROFESSOR, 工学部, 助教授 (30034383)
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| Project Period (FY) |
1987 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1988: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1987: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | Methanogen / Methanosarcina barkeri / Corrinoid / F_<430> / cytochrome / 5-Aminolevulinic acid / Fixed-bed reactor / 固定床連続培養 / 5-アミノレブリン酸 / ALA / 固定床バイオリアクター / 2-オキソグルタン酸 / 4、6-ジオキソヘプタン酸 / ビタミンB-12 / F430 / δ-アミノレブリン酸 / コバルト欠除コリノイド / テトラピロール化合物 / ヘム |
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| Research Abstract |
A methanogen, Methanosarcina barkeri contains three tetrapyrroles, i.e., Cocontaining corrinoids, Ni-containing factor F_<430> and Fe-containing cytochroms (hemes) which are biosynthesized via 5-aminolevulinic acid(ALA). In this work, the selective formation of these tetrapyrroles and ALA, a potent herbicide were carried out. The results obtained were as follows.
1. Effects of Co^<2+>, Ni^<2+>, Fe^<2+> on the selective synthesis of respective tetrapyrroels were examined in batch culture. The increase of Co^<2+> in the medium fixing the low concentrations of Ni^<2+> and F^<2+> in the medium significantly stimulated the extracellular corrinoids syntheses, while F_<430> and heme were kept almost unchanged. Similar stimulative synthesis of extracellular F_<430> or heme was observed as a function of increasing Ni^<2+> or Fe^<2+> in the medium suggesting that the syntheses of these three tetrapyrroles might be regulated with the respective matal. Then, continuous production of tetrapyrroles i
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n a fixed-bed reactor(supporter: porous diatomaceous clay) was carried out a a dilution rate, 10.1 day^<-1>. The effluent discharged from the top of reactor contained the excreted tetrapyrroles whose concentration was depended upon Co^<2+>, Ni^<2+>, Fe^<2+> in the feed medium. The maximum productivies from the roactor(1-1 bases) were 52 m [corrinoids]/day, 24 m[F_<430>]/day and 8 m [heme]/day.
2. To accumulate ALA into the culture medium, some ALA analogues were tested as inhibitor of ALA dehydratase. It was found that ALA was accumulated when levulinic acid or 4,6-dioxoheptanoic acid was added, the latted was more effective even at a much lower concentration. It was found that 2-oxoglutarate reacted as a precursor on the formation of ALA indicating that the production ALA increased 83 m from 37 m with the addition of 30 mM of 2-oxoglutarate compared with the control experiment. The continuous productions of ALA from methanol in fixed-bed reactors was tested indicating that the maximum production rate was 400 m/day. Less
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Report
(3 results)
Research Products
(15 results)
