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Structure Determination of Sweetness Inhibiting Substance and Taste modifying Protein and Mechanism of those taste-modifiers

Research Project

Project/Area Number 62560122
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 製造化学・食品
Research InstitutionYokohama National University

Principal Investigator

KURIHARA Yoshie  Yokohama National Uni., Fac. of Education, 教育学部, 教授 (90017715)

Co-Investigator(Kenkyū-buntansha) MASATOSHI Asami  Yokohama National Univ., Fac. of Education, 教育学部, 助教授 (20134439)
Project Period (FY) 1987 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1988: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1987: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsGymnemic acid / Ziziphin / miraculin / ミラクリンの一次構造 / ミラクリンの糖組成 / ミラクリン / 甘味抑制物質 / ジジフィン / ギムネマ酸
Research Abstract

Ziziphin, antisweet principle in leaves of ziziphus jujuba, was purified by silica gel chromatographuy, reverse phase chromatography and gel filtration. The chemical structure of ziziphin was established on the basis of spectral and chemical evidence. The leabes of gymnema sylvestre contain gymnemic acid which has antisweet activity. We have succeeded to isolate two major constituents of gymnemic acids by HPLC and setermined their structures. It was suggested that acyl groups in both molecules play an importnt role in generation of the antisweet activity.
New isolation method of taste-modifying protein (miraculin) from miracle fruit was established. The purified miraculin thus obtained gave a single sharp peak in reverse phase high performance liquid chromatography, indicationg that it is highly pure. The sample also gave a single band having molecular weight 28,000 in sodium dodecyl sulfate-polyacrylamido gel electrophoresis. The complete amino acid sequence of miraculin was determined by an automatic edman degradation method. miraculin was a single polypeptide with 191 amino acid residues. The calculated molecular weight based on the amino acid sequence and the carbohydrate content (13.9%) was 24,600. ASN-42 and ASN-186 were linked N-glycosidically to carbohydrate chains which consisted of glucosamine, mannose, galactose, xylose and fucose in a molar ratio of 3.03:3.00:0.69:0.96:2.12.

Report

(3 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] S.Theerasilp,et al.: J.Biol.Chem.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] S.Theerasilp,et al.: J.Biol.Chem.263. 11536-11539 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] S.Theerasilp,et al.: J.Biol.Chem.264. (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] S.Nakajo,et al.: Chem.Senses. 13. 663-669 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Y.Kurihara,et al.: Tetrahedron. 44. 61-66 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] M.Maeda,et al.: Tetrahedron Lett.(1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] S. Theerasilp; H. Yamashita; Y. Kurihara: "Purification and Partial Structure Characterization of a New Type of Sweet Protein Having Taste-modifying Action, Curculin" J. Biol. Chem.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] S. Theerasiip; Y. Kurihara: "Complete Purification and Characterization of the Taste-modifying Protein, Miraculin, from Miracle Fruit" J. Biol. Chem.263. 11536-11539 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] S. Theerasilp; H. Hitotsuya; S. Nakajo; K. Nakaya; Y. Nakamura; Y. Kurihara.: "Complete Amino Acid Sequence and Structure Characteri-zation of Taste-modifying Protein, Miraculin" J. Biol. Chem.264. (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] S. Nakajo; S. Theerasilp; K. Nakaya; Y. Nakamura; Y. Kurihara: "A Quantitative Enzyme Immunoassy for Miraculin in Miracle Fruit" Chem. Senses. 13. 663-669 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Y. Kurihara; K. Ookubo; H. Tasaki; H. Kodama; Y. Akiyama; A. Yagi; B. P. Halpern: "Studies on the Taste Modifiers. I. Purification and Structure Determination of Sweetness Inhibiting Substance in Leaves of Ziziphus jujuba" Tetrahedron. 44. 61-66 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] S.Theerasilp et al.: J.Biol.Chem.264. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] S.Theerasilp et al.: J.Biol.Chem,. 263. 11536-11539 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] S.Nakajo et al.: Chem.Senses. 13. 664-669 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] M.maeda et al.: Tetrahedron Lett.(1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Y.Kunihara et,al: Tetrahedron. 44. 61-66 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] S.Theerasilp et al.: J.Biol.Chem.

    • Related Report
      1988 Annual Research Report
  • [Publications] Y. Kurilarn et al.: Tetrahedron. 44. 61-66 (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] 中条: Proceedings of the 21st Japanese Symposium on Taste and Smell. 21. 222-226 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 前田: Proceedings of the 21st Japanese Symposium on Taste and Smell. 21. 227-230 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Therrasilp and Kurihara: J. Biological Chemistry. (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] Nakajo et al.: Biochim, Biophys. Acta. (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] M. Maeda and Kusihara: Tetrahedron. (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] Editors; Stemphen D. Roper: "Olfaction and Taste IX Annals of the New York Academy of Sciences Vol. 510" The New York Academy of Sciences, 747 (1987)

    • Related Report
      1987 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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