• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Design for the production of "Food Saccharide" materials: An approach by the practical use of enzyme function.

Research Project

Project/Area Number 62560127
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 製造化学・食品
Research InstitutionKyoto University

Principal Investigator

OHNISHI Masatake  Dept. of Food Science and Technology, College of Agric., 農学部, 助手 (90026576)

Project Period (FY) 1987 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1988: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1987: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsFood saccharide / Practical use of enzyme function / Immobilization of enzymes / Enzymes related with saccharide / アミラーゼと糖質との相互作用 / 速度論的平衡論的解析
Research Abstract

1, Streptomyces xylose isomerase (XIase) was purified with the ammonium sulfate fractionation and the DEAE Sephadex column chromatography to be pure in the electrophoretic analysis. With this enzyme preparation, the kinetic parameters, Km and kO were evaluated for some saccharides, eq. glucose, -glucose and fructose. -Glucose was found to be not a substrate. For the kinetic studies, a new method on the observation of the Xiase-catalyzed-reaction was developed on the basis of optical rotation caused by the reaction, glucose fructose, which was continuously recorded on a union automatic digital polarimeter. The optical rotation stopped-flow apparatus was also developed to measure the transient phase (within 1 sec) of the Siase reaction.
2, Interaction of Asp. niger glucoamylase with maltose and isomaltose was observed with kinetic and static methods in the presence or absence of glucose and gluconolactone, and kinetic and static parameters were evaluated for prediction of the binding subsites of these saccharides.
3, Rhizopus blucoamylase and Bacillus liquefying -amylase were immobilized to formyl-cellulofine carrier. These immobilized enzyme preparations were confirmed to be useful for the production of glucose. The latter produces -glucose, which is the substrate of isomerization by XIase. Thus, it will be possible to construct a system with co-immobilized enzymes for the direct production of fructose from starch. A new method for the determination of the liquefying -amylase-catalyzed activity was also developed on the basis of the use of a commercial glucose-kit and maltohexaose as a substrate. This method is very useful for the kinetic studies of the enzyme.

Report

(3 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • Research Products

    (37 results)

All Other

All Publications (37 results)

  • [Publications] 大西正健: 澱粉科学. 34. 58-65 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ohnishi,M.: Carbohydr.Res.165. 155-160 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 大西正健: 醗酵工学会誌. 65. 542-543 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 大西正健: 日本農芸化学会誌. 62. 96-97 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ohnishi,M.: Carbohydr.Res.161. 257-263 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ohnishi,M.: Carbohydr.Res.172. 164-169 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ohnishi,M.: Arch.Biochem.Biophys.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ohnishi,M.: Carbohydr.Res.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ohnishi,M.: Carbohydr.Res.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ohnishi,M.: Anal.Biochem.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ohnishi,M.: Carbohydr.Res.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 大西正健: 日本農芸化学会誌. 63. (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 大西正健: "酵素反応速度論実験入門" 学会出版センター, 218 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 大西正健: "酵素科学ー食品からのアプローチ" 啓学出版, 288 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 大西正健: "実験でみる生化学" 共立出版, 320 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ohnishi, M.: "Studies on the active site structure of glucoamylase" J. Jap. Soc. Starch Sci.34. 58-65 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ohnishi, M.: "Characterization, by the binding of D-mannonolactone, of the subsites adjacent to the catalytic site of glucoamylase from Rhizopus niveus" Carbohydr. Res.165. 155-160 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ohnishi, M.: "A flow-calorimetric study of the binding of iodine to amylodextrin fractions" Carbohydr. Res.161. 257-263 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ohnishi, M.: "Iodine binding to amylodextrin fractions studied by the difference spectrophotometry and potentiometry" Carbohydr. Res.172. 164-169 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ohnishi, M.: "Stopped-flow chemical modification with N-bromosuccinimide: A good probe for change in microenvironment of Trp 62 of chicken egg-white lysozyme" Arch. Biochem. Biophys.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ohnishi, M.: Enzyme Sciences---An approach based on foods. Keigaku Shuppan, 288 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ohnishi,M.: Carbohydr.Res.172. 164-169 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Ohnishi,M.: Arch.Biochem.Biophys.(1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Ohnishi,M.: Carbohydr.Res.(1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Ohnishi,M.: Carbohydr.Res.(1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Ohnishi,M.: Anal.Biochem.(1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Ohnishi,M.: Carbohydr.Res.(1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 大西正健: "酵素科学ー食品からのアプローチ" 啓学出版, 0-288 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 大西正健: "実験でみる生化学" 共立出版, 0-320 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 大西正健: 澱粉科学. 34. 58-65 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Ohnishi, M.: Carbohydr. Res.165. 155-160 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 大西正健: 日本農芸化学会誌. 62. 96-97 (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] 大西正健: 醗酵工学会誌. 65. 542-543 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Ohnishi, M.: Biochim. Biophys. Acta.

    • Related Report
      1987 Annual Research Report
  • [Publications] 大西正健: 日本農芸化学会誌. 62. 344 (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] 大西正健: "酵素反応速度論実験入門" 学会出版センター, 218 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 大西正健: "酵素科学" 啓学出版, 294 (1988)

    • Related Report
      1987 Annual Research Report

URL: 

Published: 1987-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi