Production of Chimeral Thalli by Protoplast Fusion in Porphyra yezoensis and P.tenera
| Project/Area Number |
62560196
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General fisheries
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| Research Institution | Mie University (1988)
Kyushu University (1987) |
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Principal Investigator |
ARAKI Toshiyoshi Associate professor ・ Faculty of Bioresources, Mie University, 生物資源学部(水産学部), 助教授 (40091368)
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| Project Period (FY) |
1987 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1988: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1987: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | -1,4-Mannanase / -1,3-Xylanase / Porphyranase / Protoplast / Cell fusion / Chimera / Porphyra tenera / キメラ / β-1,3-キシラナーゼ / β-1,4-マンナナーゼ |
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| Research Abstract |
Cell fusion is the superior technique to improve the quality of plants. In this study was tried to produce chimeral thallus from a red sea algae, Porphyra, by cell fusion. For carrying out cell fusion it is necessary to produce a number of protoplasts. We searched bacteria capable of degrading the cell wall of algae from natural habitats, and isolated 3 strains of bacteria which could produce a lot of cell wall-degrading enzymes ( -1,4-mannanase, -1,3-xylanase, and porphyranase). A green thallus of the spontaneous mutant was also obtained from the wild type of P.tenera. A number of protoplasts from thalli of the wild type of . Yezoensis and the green type mutant of P.tenera by the treatment with -1,4-mannanase, -1,3-xylanase, and porphyranase. These protoplasts were induced to fuse by exposing them to polyethylene glycol (PEG). The PEG-treated protoplasts developed into calli of irregular shapes, and a large nummber of plantlets were formed from the calli. These plantlets grew into sectorially variegated chimeral thalli (chimera-I) composed of brown and green sectors, in addition to brown or green thalli. Microscopical observation revealed that green sector of chimera-I turned into brown mature cells. Some monospores released from chimera-I grew into chimeral thalli composed of brown and green, and others bedame brown or green thalli. A chimeral thallus developed from monospores of chimera-I released carpospores. These carpospores grew into conchocelis and produced conchospores. A 38.4 % of the conchospores grew into chimeral thalli composed of brown and green. Therefore, chimera-I was supposed to be a thallus developed from fusion cell of P.yezoensis and P.tenera.
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Report
(3 results)
Research Products
(10 results)
