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Studies on in vitro maturation, fertilization and developmental capacity of bovine oocytes cultured in intact follicles

Research Project

Project/Area Number 62560255
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 畜産学(含草地学)
Research InstitutionObihiro University of Agriculture and Veterinary Medicine

Principal Investigator

FUKUI Yutaka  Obihiro University of Agriculture and Veterinary Medicine: Associate Professor, 畜産学部, 助教授 (40120547)

Project Period (FY) 1987 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1988: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1987: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsBovine oocytes / In vitro fertilization / development / 卵胞培養
Research Abstract

In the first year (1987), the fertilization and cleavage of bovine oocytes matured by intra- or extra-follicular methods were invesigated. Cocytes were fertilized in vitro or in the rabbit oviduct and cleavage was assessed after in vitro and in vivo (rabbit oviducts) culture of in vitro fertilized and xenogenously fertilized oocytes, respectively. The intra-follicular method did not increase the nuclear maturation rate as compared with the extra-follicular method (57.9 and 52.7%). However, the proportions of in vivo fertilized eggs (49.3%) and of cleaved eggs (34.6%) in the rabbit oviduct were higher (p<0.025) in the intra-follicular oocytes than those of the extra-follicular oocytes. These results demonstrated that the intra-follicular method of bovine oocytes provided a physiological environment for cytoplasmic maturation leading to higher fertilizability and cleavage than the conventional culture of extra-follicular oocytes.
In the second year (1988), a co-culture system for oocyte maturation was established using granulosa cells in media (TCM 199 + 10% estrous cow serum). In vitro fertilization rate was improved by the use of swim-up and heparin treatment in frozen-thawed bovine spermatozoa (50 -60%). Presently, I have obtained rates of in vitro maturation (60 -70%), fertilization (50 -60%), cleavage (50%) and development into blastocysts (10 -20%) . For development of in vitro fertilized eggs, bovine oviduct epithelial cells were sucssesfully employed in media. One of three heifers received thransfer of in votro developed blastocysts was pregnant, and has been expecting the calving in march 23th, 1989 at the Obihiro University Farm.

Report

(2 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Fukui,Y,;Imai,K.,;Alfonso,N.F.;Ono,H.: Journal of Animal Science. 64. 935-941 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 福井豊、田辺亮一、青柳敬人、小野斉: 帯広畜産大学学術研究報告. 15. 9-15 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 青柳敬人・福井豊・ら: 家畜繁殖技術研究会誌. 10. 1-3 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Fukui,Y.;Ono,H.: Veterinary Record. 122. 282 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Fukui,Y.;Urakawa,M.,;Sasaki,C.,;Chikamatsu,N.;Ono,H.: Animal Reproduction Science.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Fukui,Y.: Journal of Animal Science.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Fukui,Y.;Ono,H.: Journal of Reproduction and Fertility.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 金川弘司、高橋芳幸、井上忠恕、福井豊: "牛の受精卵(胚)移植〈第2版〉" 近代出版, 142-148 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Fukui, Y.; Imai, K.; Alfonso, N.F.; Ono, H.: "Follicle culture enhances fertilizability and cleavage of bovine oocytes matured in vitro" Journal of Animal Science. 64. 935-941 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Fukui, Y.; Tanabe, R.; Aoyagi, Y.; Ono, H.: "Effect of culture time of follicle-enclosed bovine oocytes for in vitro maturation and fertilization" Research Bulletin of Obihiro University, Series I. 15. 187-193 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Fukui, Y.; Ono, H.: "In vitro development to blastocyst of in vitro matured and fertilized Bovine oocytes" Veterinary Record. 122. 282 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Fukui, Y.; Urakawa, M.; Sasaki, C.; Chikamatsu, N.; Ono, H.: "Develpoment to late morula or blastocyst in vivo and in vitro following in vitro fertilization of bovine oocytes matured in vitro" Animal Reproduvtion Science (1989: in printing).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Fukui, Y.: "Effect of sera and steroid hormones on development of bovine oocytes matured and fertilized in vitro and co-cultured with bovine oviduct epithelial cells" Journal of Animal Science (1988: accepted).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kanagawa, H.; Takahashi, Y.; Inoue, T.; Fukui, Y.: Embryo Transfer in Cattle (in Japanese). Kindai-Publishing Co., Tokyo, Japan, 142-148 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Fukui,Y.;Imai,K.;Alfonso,N.F.;Ono,H.: Journal of Animal Science. 64. 935-941 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] 福井豊,田辺亮一,青柳敬人,小野斉: 帯広畜産大学学術研究報告. 15. 9-15 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] 青柳敬人,福井豊 他: 家畜繁殖技術研究会誌. 10. 1-3 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Fukui,Y.;Ono,H.: Veterinary Record. 122. 282 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Fukui,Y.;Urakawa,M.;Sasaki,C.;Chikamatsu,N.;Ono,H.: Animal Reproduction and Science.

    • Related Report
      1988 Annual Research Report
  • [Publications] Fukui,Y.: Journal of nimol Science.

    • Related Report
      1988 Annual Research Report
  • [Publications] Fukui,Y.;Ono,H.: Journal of Reproduction and Fertility.

    • Related Report
      1988 Annual Research Report
  • [Publications] 金川弘司,高橋芳幸,井上忠恕,福井豊: "牛の受精卵(胚)移植<第2版>" 近代出版, 142-148 (1988)

    • Related Report
      1988 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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