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Efficient use of oocytes by the perfusion of farm animal ovary

Research Project

Project/Area Number 62560267
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 畜産学(含草地学)
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

MIYAMOTO Hajime  Faculty of Agriculture, Kyoto University, Professor, 農学部, 教授 (00026618)

Co-Investigator(Kenkyū-buntansha) SATO Eimei  Faculty of Agriculture, Kyoto University, Associate Professor, 農学部, 助教授 (80093243)
ISHIBASHI Takehiko  Faculty of Agriculture, Kyushu Tokai University, Professor, 農学部, 教授 (20031156)
Project Period (FY) 1987 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1989: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 1988: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1987: ¥1,300,000 (Direct Cost: ¥1,300,000)
Keywordsfarm animal ovary / ovarian function / Graafian follicle / ovulation / perfusion / medium / ovarian histology / ovarian histochemistry / 培養装置 / グラーフ卵胞 / 組織学 / ヤギ卵巣 / 卵細胞の成熟 / pH / ブドウ糖
Research Abstract

A technique for in vitro perfusion of goat ovaries is studied to use oocytes efficiently and to examine ovarian function. The effect of perfusion with different media on ovarian function and, in particular, on the phenomenon of follicular rupture and swelling is examined. A system has been developed for the perfusion of the goat ovary in vitro. The perfusion apparatus consisted of a recycling system with three major portions: an oxygenator, a roller pump, and a perfusion chamber. The functional integrity of the perfused ovary is confirmed by serial determinations of the perfusate pH, glucose and lactate concentrations, and by histology and histochemistry of the ovary.
After several preliminary experiments, nine experiments, where the ovary containing Graafian follicles was used, were tried. In one of nine experiments the rupture of two Graafian follicles occurred by perfusion of the goat ovary. However in the other experiments the rupture of Graafian follicles did not occur. Perfusion o … More f the ovary caused swelling of Graafian follicles and small antral follicles, accompanied by a stromal edema. Graafian follicular rupture observed in perfusion system may be artifacts and careful selection of the perfusion medium is needed to avoid misinterpretation.
Glucose consumption and lactate production occurred continuously during the 6-hour perfusion period. It was shown by light microscopy that almost normal ovarian structure was maintained throughout the perfusion, although interstitial edema formation was noted in the perfused ovaries. In the histochemical study, normal activity of 3beta-HSD and G6PDH was maintained in the theca interna and granulosa cells of follicles in the perfused ovaries. In contrast, electron microscopic observations indicated that the present perfusion system is not satisfactory to maintain ovarian tissue.
In conclusion, a perfusion system of the farm animal ovary is useful for the efficient use of oocytes and for the study of ovarian function if perfusion technique could be improved. Less

Report

(4 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • 1987 Annual Research Report
  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] 宮本元: "ヤギ卵巣の還流培養装置の作製" 家畜繁殖技術研究会誌. 11. 24-27 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 宮本元: "還流培養したヤギ卵巣の組織学的検討" 家畜繁殖技術研究会誌. 12. 19-23 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] H.Miyamoto: "Perfusion of the goat ovary in vitro" Biology of Reproduction.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] H. Miyamoto: "A trial manufacture of perfusion apparatus of goat ovary" Jpn. J. Animal Reproductive Technology 11: 24-27.1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] H. Miyamoto: "Histological observations on the goat ovary perfused in vitro" Jpn. J. Animal Reproductive Technology 12: 19-23.1990.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] H. Miyamoto: "Perfusion of the goat ovary in vitro" Biology of Reproduction.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 宮本元: "還流培養したヤギ卵巣の組織学的検討" 家畜繁殖技術研究会誌. 12. 19-23 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] H.Miyamoto: "Perfusion of the goat ovary in vitro" Biology of Reproduction.

    • Related Report
      1989 Annual Research Report
  • [Publications] 宮本元: 家畜繁殖技術研究会誌. 11. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 宮本元: 家畜繁殖技術研究会誌.

    • Related Report
      1987 Annual Research Report
  • [Publications] 宮本元: 日本畜産学会報.

    • Related Report
      1987 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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