| Project/Area Number |
62560279
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
畜産化学
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| Research Institution | TOHOKU UNIVERSITY |
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Principal Investigator |
SUYAMA Kyozo Associate Professor Tohoku University, Faculty of Agricultuutre, 農学部, 助教授 (70005635)
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| Co-Investigator(Kenkyū-buntansha) |
SUGAWARA Hiroshi Asistant Fac. Agriculture, Tohoku Univ., 農学部, 教務職員 (80089797)
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| Project Period (FY) |
1987 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1988: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1987: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | lactulose / galacto-oligosaccharides / Bifidus activity / 糖転移反応 / ビフィダス活性 / ガラクトシル転移反応 / オリゴ糖 / β-ガラクトシダーゼ |
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| Research Abstract |
Lactulose has acquired a broad range of usefulness; bifidus factor in maintaining the normal intestinal microflora of infant and also adult, reduction the frequency of recurrences of encephalophthy and permits increased dietary protein consumption in the great majority of such patients, less well defined severe exacerbations of nitrogenous portal-systemic encephalopathy.
In this report, isolation and characterization of oligosaccharides formed by the transgalactosylation reaction of lactulose catalyzed by Aspergillus oryzae -galactosidase were described. Major Tri-, tetra- and pentasaccharide has been isolated by charcoal column and preparative paper chromatography and shown to have the structure: O- -D-galactopyranosyl-(1---6)-O- -D-galacto-pyranosyl-(1---4)-D-fructose(TR), O- -D-galactopyranosyl-(1---6)-O- -D-galacto-pyranosyl-(1---6)-O- -D-galactopyranosyl-(1---4)-D-fructose(TE), and O- -D-galactopyranosyl-(1---6)-O- -D-galactopylanosyl-(1---6)-O- -D-galactopyranosyl-(1---6)-O- -D-galactopyranosyl-(1---4)-D-fructose(PE).
In attempt to produce oligosaccharides in medium containing lacturose, an Aspergillus oryzae S-65 screened from 25 aspergillus species and 24 Aspergillus oryzae strains accumulate in large quantities of galacto oligosaccharides from lactulose. The most abundant oligosaccharide formed were TR and TE. A Cryptococcus laurentii also produced oligosaccharides in lacturose containing medium. The yield of trisaccharides was 15.5 per cent based on total saccharides. The main product formed was A hitherto undescrided trisaccharide, O- -D-galactopyranosyl-(1---4)-O- -D-galactopyranosyl-(1---4)-D-fructose.
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