| Project/Area Number |
62560295
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
基礎獣医学
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| Research Institution | Kagoshima University |
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Principal Investigator |
SATO Heiji Kagoshima Univ., Fac.of Agriculture, Professor, 農学部, 教授 (80041672)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMIZU Tsutomu Kagoshima Univ., Fac.of Agriculture, Professor, 農学部, 教授 (50072921)
MOCHIZUKI Masami Kagoshima Univ., Fac.of Agriculture, Assoc.Prof., 農学部, 助教授 (90157834)
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| Project Period (FY) |
1987 – 1989
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| Project Status |
Completed (Fiscal Year 1989)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1989: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 1988: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1987: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | L-PHASE OF BACTERIA / BACTERIAL L-FORM / CELL-WALL DEFECTIVE BACTERIA / SPHEROPLAST / PROTOPLAST / CELL-WALL DEFECTIVE BACTERIA / Spheroplast / Protoplast / 細胞壁表失細菌 / 細胞壁喪失菌 / 細胞壁 / 薬剤耐性 / 関節炎 / アジュバント関節炎 / cell wall less bacteria |
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| Research Abstract |
Induction of L-forms were attempted from many gram positive and Gram negative strains using Methocillin, lysozyme or glycine. In vivo induction was also attempted in mice and eggs. The characteristics of L-forms were compared with parent intact forms and following results were obtained.
1. Resistance to the antibiotics disturbing cell-wall-formation increased in both stable and unstable L-forms, on the other hand, inhibitory effect of the antibiotics disturbing the cytoplasmic protein synthesis was not similar to the case of L-forms.
2. SDS-PAGE analysis of structural proteins detected a remarkable difference between L-forms and intact forms in Staphylococcus aureus and Pseudomonas aeruginosa L-forms. This fact indicate that the structural component and or metabolism of L-forms are not the same one as those in their parent intact cells.
3. Agglutination test between L-forms and intact forms were performed using Staph.aureus and Ps.aeruginosa. Similar results were obtained in both species that L-forms cell antigen not react with intact cell antibody but react with homologous L-forms antibody while intact cell antigen react both antibody.
4. No pathogenicity was proved by Staph.aureus stable L-forms in mice, however, the L-forms increased pathogenicity of Fusobacterium necrophorum when they are mixed. Reverted incomplete coccal forms were observed in peritoneal fluid of mix-infected mice.
5. Induction of L-forms from Salmonella pullorum were tested in vitro and in incubating eggs. It is note worthy that strains which changeable to L-forms in vitro were easily transformed into L-forms without any inducing agent in eggs.
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