Localization of angiotensinogen and its mRNA in hepatocytes of adult and embryonic rats.
| Project/Area Number |
62570003
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | University of Tsukuba |
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Principal Investigator |
UCHIYAMA Yasuo Department of Anatomy, Institute of Basic Medical Sciences, 基礎医学系, 助教授 (10049091)
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| Co-Investigator(Kenkyū-buntansha) |
MIYAZAKI Hitoshi Institute of Applied Biological Chemistry, 応用生物化学系, 講師 (40183636)
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| Project Period (FY) |
1987 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1988: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1987: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Rat / Hepatocytes / Angiotensinogen / Circadian variation / Pre- and Post-natal development / 生後発達 / 免疫組織化学 / 抗アンギオテンシノーゲン / アンギオテンシノーゲン及び同mRNAの肝細胞内局在に関する研究 / mRNA / insitnハイブリダイゼーション |
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| Research Abstract |
To examine localization of angiotensinogen (ANG) and its mRNA in hepatocytes of adult and embryonic rats, in situ hybridization and immunocytochemistry were applied to liver tissue. In in situ hybridization used, specific as well as non-specific signals appeared in liver tissue, so that the present study was performed mainly using immunocytochemistry. For this, we purified ANG from nephrectomized rat plasma, and prepared anti-ANG, whose specificity was confirmed by immunoblotting, radioimmunoassay and immunocytochemistry. By immunocytochemistry, a clear-cut variation in immunoreactivity for ANG was detected in adult rat hepatocytes over 24 h; it was strong early in the light period, whereas it was weak early in the dark period. The drastic variation in the reactivity was seen especially in periportal hepatocytes. The change in immunoreactivity for ANG in hepatocytes over 24 h is similar to that in quantity of the rough endoplasmic reticulum in hepatocytes (Uchiyama and Asari 1984); ANG content in hepatocytes is high, when proteim synthesis of hepatocytes is active. As for pre- and postnatal development, ANG appeared faintly in hepatocytes at 17 days of gestation, while at 20 days, immunoreactivity for ANG became vivid. At birth, the strongest immunoreactivity was detected in hepatocytes, while the reactivity in hepatocytes after 5 postnatal days became similar to that of adult hepatocytes. The result that ANG content in hepatocytes increases from 17 to 20 days of gestation is similar to that of renin content in juxtaglomerular cells. This indicates that ANG as the renin substrate participates in the renin-angiotensin system at the end of fetal life, which regulates blood pressure.
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Report
(3 results)
Research Products
(12 results)
