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Control of the distribution of acetylcholine receptor in muscle cells

Research Project

Project/Area Number 62570013
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General anatomy (including Histology/Embryology)
Research InstitutionUniversity of the Ryukyus

Principal Investigator

ISHIKAWA Yuji  University of the Ryukyus Department of Anatomy Associate Professor, 医学部, 助教授 (20110299)

Project Period (FY) 1987 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1989: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1988: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1987: ¥900,000 (Direct Cost: ¥900,000)
KeywordsMUSCLE / NERVE / RECEPTOR / ACETYLCHOLINE RECEPTOR / ニワトリ / ラット / メダカ
Research Abstract

In order to determine the roles of nerves in the formation of clusters of acetylcholine receptors (AChRs) during synaptogenesis, we examined the distribution of AChRs in denervated, nerve-transplanted (neurotized) muscles and in regenerated skeletal muscles of adult chickens by fluorescence microscopy using curaremimetic toxins. In the denervated muscles, many extrajunctional clusters developed at the periphery of some of the muscle nuclei of a single muscle fiber and continued to be present for up to 3 months. The AChR accumulations originally present at the neuromuscular junctions disappeared within 3 weeks. In the neurotized muscles, line-shaped AChR clusters developed at 4 days after transection of the original nerve, but no change in the distribution of AChRs had occurred even at 2 months after implantation of the foreign nerve. The line-shaped AChR clusters were found to be newly formed junctional clusters as they were associated with nerve terminals of similar shape and size. Some of both the line-shaped and extrajunctional clusters were formed at least partly by the redistribution of preexisting AChRs. Finally, based on the above observations, the regenerating muscle fibers in normal muscles and in denervated muscles were examined: The extrajunctional clusters appeared in both kinds of muscles at 2 weeks after injury. Afterward, during the innervation process, the line-shaped AChR clusters developed while the extrajunctional clusters disappeared in the innervated muscles. In contrast with this, in the absence of innervation, only the extrajunctional clusters continued to be present for up to 3 months. These results demonstrate clearly that the nerve not only induces the formation of junctional clusters at the contact site, but also prevents the formation of clusters at the extrajunctional region during synaptogenesis.

Report

(4 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • 1987 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] A.Arakaki: "An experimental study on the direct nerve implantation in the anterior tibial muscle of the rat,1st report." Ryukyu Med.J.10. 27-33 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] A.Arakaki: "An experimental study on the direct nerve implantation in the anteriortobsal muscle of the rat,2nd report." Jpn.Soc.Surg.Hand. 4. 39-45 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Y.Ishikawa: "Effect of innevation on the distribution of a cetylcholive receptors in regenerating skeletal muscles of adult chickens." Develop.Biol.125. 115-126 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] S.Kuratani: "Eearly development of the hypoglossal nerve in the click empryo as obzerved by the whole-mount nerve staining method." Amer.J.Anat.182. 155-168 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] S.Kuratani: "Early development of the fooial nerve in the chick embryo with special refference to the development of the chorda tympani." Amer.J.Anat.182. 169-182 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Y.Ishikawa: "Development of caudal structures of a morpho genetic mutant(Da)in the taleost fish,medaka(Dryziar,latipes)." J.Morph.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 石川裕二(分担執筆): "組織細胞化学の技術 細胞膜" 朝倉書店, 325 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] A. Arakaki, K. Ibaraki, M. Kayo, M. Takeuchi, H. Hokama, Y. Ishikawa and S. Tanaka: "An experimental study on the direct nerve implantation in the anterior tibial muscle of the rat; 1st report" Ryukyu Med.J.10. 27-33 ((1987))

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] A. Arakaki, K. Ibaraki, M. Kayo, T. Miyasato and Y. Ishikawa: "An experimental study on the direct nerve implantation in the anterior tibial muscle of the rat" 2nd report. J. Jpn. Soc. Surg. Hand, 4, 39-45 (1987).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Y. Ishikawa, A. Arakaki, N. Shimizu, K. Ibaraki and S. Tanaka: "Effects of innervation on the distribution of acetylcholine receptors in regenerating skeletal muscles of adult chickens." Develop. Biol. 125, 115-126 (1988).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] S. Kuratani, S. Tanaka, Y. Ishikawa and C. Zukeran: "Early development of the hypoglossal nerve in the chick embryo as observed by the whole-mount nerve staining method." Am. J. Anat., 182, 155-168 (1988).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] S. Kuratani, S. Tanaka, Y. Ishikawa and C. Zukeran: "Early development of the facial nerve in the chick embryo with special reference to the development of the chorda tympani" Am. J. Anat. 182, 169-182 (1988).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Y. Ishikawa: "Development of caudal structures of a morphogenetic mutant (Da) in the teleost fish, medaka (Oryzias latipes)." J. Morph.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 石川裕二: ミクロスコピア. 4. 200-201 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] 新垣晃: 琉球大学医学雑誌. 10. 27-33 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] 新垣晃: 日本手の外科学会雑誌. 4. 39-45 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] 石川裕二: Developmental Biology. 125. 115-126 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 倉谷滋: American J. Anatomy. 182. 155-168 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 倉谷滋: American J. Anatomy. 182. 169-182 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 石川裕二: 琉球大学医学雑誌. 10(1). 27-33 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 石川裕二: 日本手の外科学会雑誌. 4(1). 39-45 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 石川裕二: Developmental Biology. 125. 115-126 (1988)

    • Related Report
      1987 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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