• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Regulatory role of gamma-aminobutyric acid (GABA) in catecholamine release from adrenal medulla

Research Project

Project/Area Number 62570088
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General pharmacology
Research InstitutionHiroshima University

Principal Investigator

KITAYAMA Shigeo  Department of Pharmacology Hiroshima University School of Dentistry Instructor, 歯学部, 助手 (80177873)

Co-Investigator(Kenkyū-buntansha) TSUJIMOTO Akira  Department of Pharmacology Hiroshima University School of Dentistry Professor, 歯学部, 教授 (90034181)
Project Period (FY) 1987 – 1988
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1988: ¥600,000 (Direct Cost: ¥600,000)
KeywordsGamma-aminobutyric acid / Adrenal medulla / Chromaffin cell / Catecholamine release / Neuromodulator / Ca^<2+> / Benzodiazepine / Cl^- channel / γ-アミノ酪酸 / カテュールアミン遊離ニューロモジュレーター / 膜電位 / カテコールアミン遊離 / ニューロモジュレーター
Research Abstract

We investigated the role of gamma-aminobutyric acid (GABA) in catecholamine (CA) release from adrenal medulla using a primary culture of bovine adrenal chromaffin cells. 1. GABA elicited CA release from monolayer cultures of bovine adrenal chromaffin cells, and facilitated acetylcholine (ACh)-evoked CA release via GABA receptor. Benzodiazepines and pentbarbital enhanced the action of GABA. 2. GABA caused an increase in ^<45>Ca uptake associated with CA release. Ach-induced ^<45>Ca uptake was additively enhanced by GABA, whereas veratridine (at low concentration)- evoked ^<45>Ca uptake was more than additively enhanced by GABA. 3. GABA-evoked ^<45>Ca uptake and CA release were modified by replacing extracellular C1^- by impermeable anion or sucrose in a time-related fashion: sudden replacement of extracellular C^- by sucrose enhanced GABA-evoked CA release whereas long-term treatment with low C1^- medium reduced GABA-evoked CA release and ^<45>Ca uptake. 4. GABA increased bis-oxonol fluorescence, reflecting a depolarization of chromaffin cells. Furthermore, GABA caused an increase in cytosolic Ca^<2+> ([Ca^<2+>]i), assessed by quin2. GABA-induced depolarization and rise of [Ca^<2+>]i were well correlated with ^<45>Ca uptake and CA release.
Based on these results, we concluded that GABA causes an influx of Ca^<2+> through voltage- gated Ca^<2+> channels as a consequence of depolarization via C1^--dependent mechanism. This, in turn, elicited [Ca^<2+>]i rise leading to an exocytotic release of CA by GABA itself and to an facilitatory modulation by GABA of stimulation-evoked CA release.

Report

(3 results)
  • 1989 Final Research Report Summary
  • 1988 Annual Research Report
  • 1987 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] S.Kitayama et al.: "Benzodiazepines facilitate the stimulatory action of γ-aminobutyric acid(GABA)on basal and veratridine-evoked catecholamine release from bovine adrenal chromaffin cells." Arch.int.Pharmacodyn.Ther.300. 254-264 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] S.Kitayama et al.: "Enhancement by GABA of the stimulation-evoked catecholamine release from cultured bovine adrenal chromaffin cells." Naunyn-Schmiedeberg's Arch.Pharmacol.(1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] S.Kitayama et al.: "GABAergic modulation of catecholamine release from cultured bovine adrenal chromaffin cells.Evidence for the involvement of Cl-dependent Ca^<2+> entry." Naunyn-Schmiedeberg's Arch.Pharmacol.(1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] S.Kitayama et al.: "GABA receptor-mediated increase of cytosolic Ca^<2+> in isolated bovine adrenal chromaffin cells." Biochim.Biophys.Acta. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] S. Kitayama, K. Morita, T. Dohi, and A. Tsujimoto: "Benzodiazepines facilitate the stimulatory action of gamma-aminobutyric acid (GABA) on basal and veratridineevoked catecholamine release from cultured bovine adrenal chromaffin cells." Arch. int. Pharmacodyn. Ther.300. 254-264 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] S. Kitayama, K. Morita, T. Dohi, and A. Tsujimoto: "Enhancement by GABA of the stimulation-evoked catecholamine release from cultured bovine adrenal chromaffin cells." Naunyn-Schmiedeberg's Arch. Pharmacol.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] S. Kitayama, K. Morita, T. Dohi, and A. Tsujimoto: "GABAergic modulation of catecholamine release from cultured bovine adrenal chromaffin cells. Evidence for the involvement of Cl^--dependent Ca^<2+> entry." Naunyn-Schmiedeberg's Arch. Pharmacol.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] S. Kitayama, K. Morita, T. Dohi, and A. Tsujimoto: "GABA_A receptor-mediated increase of cytosolic Ca^<2+> in isolated bovine adrenal chromaffin cells." Biochim. Biophys. Acta.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] S.Kitayama,et al.: Naunyn-Schmiedeberg's Arch. Pharmacol.

    • Related Report
      1988 Annual Research Report
  • [Publications] S.Kitayama,et al.: Naunyn-Schmiedeberg's Arch. Pharmacol.

    • Related Report
      1988 Annual Research Report
  • [Publications] Shigeo Kitayama et al.: Naunyn-Schmiedeberg's Arch. Phrmacol.

    • Related Report
      1987 Annual Research Report
  • [Publications] Shigeo Kitayama et al.: Naunyn-Schmiedeberg's Arch. Phrmacol.

    • Related Report
      1987 Annual Research Report

URL: 

Published: 1988-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi