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Detection and purification of the promoter region-binding protein involved in the tissue-specific gene expression

Research Project

Project/Area Number 62570102
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General medical chemistry
Research InstitutionToyama Medical and Pharmaceutical University

Principal Investigator

OGAWA Hirofumi  Toyama Med. & Pharmaceutical Univ. Fac. of Medicine, 医学部, 助教授 (30111743)

Co-Investigator(Kenkyū-buntansha) FUJIOKA Motoji  Toyama Med. & Pharmaceutical Univ. Fac. of Medicine, 医学部, 教授 (30030000)
Project Period (FY) 1987 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1988: ¥300,000 (Direct Cost: ¥300,000)
KeywordsRat liver / Serine dehydratase / Cloning / Gene structure / Consensus sequences / コンセンサス配列 / アミノ酸配列 / 試験管内転写実験 / 核抽出液 / グアニド酢酸メチラーゼ / 遺伝子クローニング / プロモーター / グルココルチコイドレスポンスエレメント / GRE
Research Abstract

In thid laboratory, several genomic clones as well as cDNA clones of rat liver enzymes involved in amino acid metabolism have been isolated. Among them, serine dehydratase is the inducible enzyme, whose activity is subject to change by the treatment of various hormones or by feeding various composition of diet. To learn the molecular mechanim of enzyme induction/suppression, we attempoted to clone the serine dehydratase cDNA. One clone was screened from a gtll library carrying rat licer cDNAs. The cDNA was found to encode 363 amino acids and the expected molecular weight atrees well that obtained previously by sds/page of the purified protein. We also obtained the corresponding genomic clones and determined their DNA sequence. The serine dehydratase gene consisted of 9 exons and 8 introns, whose boundary sequences are obeyed by a gt/ag rule. The 5' flanking region of the gene contains a variety of sequences that are similar to the consensus elements which are proved to aggect the transcription of other genes. Two sequences similar to glucocorticoid response element are located at positions -2240 and -280. Since serine dehydratase is under control by glucagon and dexamethasone, the occurrence of such sequences is quiet reasonable. to prove the function of these sequences and to look for the specific factors which might regulate the gene transcription, we are developing the in vitro transcription system usin unclear extracts obtained from rat licers maintained on various diets, and the g-less cassette vector (gorski et al. 1987) having fragments of the 5' flanking region of the serine dehydratase gene.

Report

(3 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Ogawa,et al.: Proc.Natl.Acad.Sci.USA. 85. 694-698 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ogawa,H.;Fujioka,M.: Nucl.Acids Res.16. 8715-8716 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ogawa,et al.: Proc.Natl.Acad.Sci.USA. 85. 5809-5813 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ogawa,et al.: Nucl.Acids Res.16. 10921-10923 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ogawa,et al.: Biochim.Biophys.Acta.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ogawa et al.: "Molecular cloning, sequence analysis, and expression in Escherichia coli of the cDNA for guanidinoacetate methyltransferase from rat liver" Proc. Natl. Acad. Sci. USA. 85. 694-698 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ogawa, H.; Fujioka, M.: "Nucleotide sequence of the rat guanidinoacetate methytransferase gene" Nucl. Acids Res.16. 8715-8716 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ogawa et al.: "Isolation and nucleotide sequence of the cDNA for rat liver serine dehydratase mRNA and structures of the 5' and 3' flanking regions of the serine dehydratase gene" Proc. Natl. Acad. Sci. USA. 85. 5809-5813 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ogawa et al.: "Sequence of the rat serine dehydratase gene" Nucl. Acids Res.16. 10921-10923 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ogawa et al.: "The peptides sequences near the bound pyridoxal phosphate are conserved among serine dehydratase from rat liver, threonine dehydratases from yeast and Escherichia coli" Biochim. Biophys. Acta. (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ogawa et al.: Proc.Natl.Acad.Sci.USA. 85. 694-698 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Ogawa,H.;Fujioka,M.: Nucl.Acids Res.16. 8715-8716 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Ogawa et al.: Proc.Natl.Acad.Sci.USA. 85. 5809-5813 (988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Ogawa et al.: Nucl.Acids Res.16. 10921-10923 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Ogawa et al.: Biochim.Biophys.Acta.

    • Related Report
      1988 Annual Research Report
  • [Publications] Ogawa, H.他: Prec. Natl. Acad. Sci, USA. 85. 694-698 (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] Ogawa, H.他: Proc. Natl. Acad. Sci. USA.

    • Related Report
      1987 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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