| Project/Area Number |
62570106
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General medical chemistry
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| Research Institution | Osaka University |
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Principal Investigator |
NOGUCHI Tamio Osaka University Medical School , Assistant, 医学部, 講師 (70135721)
|
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| Project Period (FY) |
1987 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1988: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1987: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Pyruvate kinase isozymes / Tissue-specific expression / シス作用制御領域 |
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| Research Abstract |
Cis-acting elements responsible for expression of pyruvate kinase isozyme genes were studied by transfection ofchloramphenicol acetyltransferase fusion gene into primary cultures of hepatocytes and dRIH-84 hepatoma cells. The results suggested that the 5'-flanking region of the L-type cap site of the L gene contained cisacting element responsible for tissue-specific expression from -185bp to -152bp. This element was characteristic of enhancer and required DNA region from -94bp to -62bp for its enhancer activity. It was also suggested that the 5'-flanking region of the R-type cap site of the L gene contained cis-acting element responsible for expression of the R-type. On the other hand, the 5'-flanking region of the M gene contained two cis-acting elements. One is stimulatory element present from -286bp to -68bp and another is inhibitory region from -358bp to -286bp.
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