| Project/Area Number |
62570116
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General medical chemistry
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| Research Institution | National Cardiovascular Center Research Institute (1988)
佐賀医科大学 (1987) |
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Principal Investigator |
MUKAI Tsunehiro Department of Bioscience, バイオサイエンス部, 部長 (40108741)
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| Project Period (FY) |
1987 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1988: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1987: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | aldolase C / Purkinje cell / In Situ hybridization / in situ hybridization / 脳型アルドラーゼ / ID配列 / In Situ Hybridization |
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| Research Abstract |
Rat aldolase C gene was isolated. The gene comprises 9 exons interruped by 8 introns. The nucleotide sequence of the gene was determined, which includes 801 nucleotides(nt.) of 5'flanking, 3590 nt. of the gene region and 461 nt. of 3' flanking sequences. Two transcriptional initiation sites were also determined by the method of S1 mapping and primer extension. Aldolase C mRNA was exclusively expressed in brain and some in hepatoma. To identify the cell expressing aldolase C mRNA in brain we performed in situ hypridization of brain and spinal cord using riboprobe. We searched cerebral cortex, cerebellum, hippocampus, thalamus of the brain. Surprisingly silver grains accumulated in the Purkinje cell of the cerebellum and some in dorsal root ganglion and Nuc. motorius lateralis of the spinal cord. We also measured the copy number of the gene in the genome. From the measurement of the geme, titrated by Southern blotting, we conclude that a single copy of the rat aldolase C gene occurs per rat haploid genome.
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