| Project/Area Number |
62570139
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Jikei University School of Medicine |
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Principal Investigator |
MATSUDA Makoto (1988-1989) Jikei Univ. Biochem. Prof., 医学部, 教授 (80056506)
八木 康之 (1987) 慈恵医大, 医学部, 講師 (40174487)
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| Co-Investigator(Kenkyū-buntansha) |
YAGI Yasuyuki Jikei Univ. Biochem. Prof. assist., 医学部, 講師 (40174487)
MIZUNO Aritake Jikei Univ. Biochem. Prof. assoc., 医学部, 助教授 (40056966)
松田 誠 慈恵医大, 医学部, 教授 (80056506)
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| Project Period (FY) |
1987 – 1989
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| Project Status |
Completed (Fiscal Year 1989)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1989: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1988: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1987: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Optic nerve transection / Nerve regeneration / Bullfrog / Tubulin / Actin / Messenger RNA / Hybridization / Retina / 神経切断 / チュ-ブリン / 視神経再生 / チューブリン / グロースコーン / チューブリンメッセンジャーRNA / ウシガエル視神経 |
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| Research Abstract |
The neurons of mammalian central nervous system ( CNS ) show little or no functional regeneration after injury. On the contrary, in lower vertebrate ( frog and fish ) the neurons of optic nerve can regenerate. In this study, we investigated the specific gene expression in the retinas of bullfrog that optic nerve was transected. We confirmed the optic nerve regeneration by the microscopic examination and the recovery of axonal transport. Northern and dot hybridizations showed the specific gene expression as follows.
1) In the retina tubulin mRNA increased to a maximum at 1-2 hours after optic nerve transection with no specific change of actin mRNA. 2) For 2-28 days after transection, tubulin mRNA increased gradually after the rapid and transient increase and actin mRNA increased to a maximum at 7 days ( more than twofold compared to the control retinas ). 3) No significant changes were detected on the mRNA levels of some onco- genes and ornithine decarboxylase for 1-4 hours after transection. To identify and clone unknown genes induced by optic nerve transection, the new method for cloning the specific gene by polymerase chain reaction ( PCR ) method is under investigation.
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