| Project/Area Number |
62570158
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Experimental pathology
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
NAITO Yasuhisa First Department of Pathology, Hamamatsu University School of Medicine(Assistant), 医学部, 助手 (10107815)
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| Project Period (FY) |
1987 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1988: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1987: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | in vitro / gastric scirrhous cancer cell / diploid fibroblast / growth factor / コラーゲン産生促進因子 / コラーゲン / 線維芽細胞 / collagen / 線維芽細胞増殖因子 / collagen産生促進因子 |
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| Research Abstract |
To elucidate the mechanisms of increase of stromal collagen in scirrhous carcinoma of the stomach, it was demonstrated that gastric cancer cells(MKN-45 and MKN-28), including those of scirrhous cancer(KATO-III) secreted new fibroblast growth factor and collagen promoting factor participated with collagen production by human diploid skin fibroblasts(HSF-1) in culture. these factors were biochemically characterized as follows.
(1) Fibroblast growth factor: The molecular weight of crude material(disignated as new FGF) estimated by gel filtration on Sephadex G-75 was found to be about 8,000. In order to determine the proportion of growth promoting activity, the [^3H]thymidine incorporation assay was performed with HSF-1 cells as target cells. The rate of cell proliferation enhanced by this factor is approximately three to four time. There were no statistical differences in the potencial growth promoting activity among new FGFs secreted by three gastric cell lines. On isoelectric focusing analysis, this factor gave a major peak at pH 4.3 and a minor peak at pH 5.0.
(2) Collagen promoting factor: The fraction estimated by gel filtration on Bio-Gel P-60 promoted collagen production in HSF-1 cells with [^3H]proline incorporation assay. The rate of collagen production promoted by this factor is approximately 3 times. Moreover,MRK-49F cells showed colony stimulating activity in soft agar medium containing EGF(2ng/ml). This factor also were completely neutralized the collagen producing activity in fibroblasts by anti-TGF- antibody.
This finding suggests that the mechanism by which the fibrous stroma is produced in scirrhous cancer is complicated and may be affected by so-called fibroblast growth factor and collagen promoting factor secreted by gastric scirrhous cancer cells.
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