| Project/Area Number |
62570188
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
細菌学
|
|---|
| Research Institution | Osaka University Medical School |
|---|
Principal Investigator |
DOHI Yoshitane Associate Professor, Department of Bacteriology,Osaka University Medical School, 医学部, 助教授 (10028574)
|
|---|
| Project Period (FY) |
1987 – 1988
|
| Project Status |
Completed (Fiscal Year 1988)
|
| Budget Amount *help |
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1988: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1987: ¥1,600,000 (Direct Cost: ¥1,600,000)
|
|---|
| Keywords | bacterial glycolipid / 免疫抑制 / 癩菌 / Phenolic glycolipid-1 |
|---|
| Research Abstract |
Various bacterial products have positive or negative effects on the immunological resistance of the host. Profound immunological suppression is observed in patients with lepromatous leprosy, in whose sera a unique glycolipid to Mycobacterium leprae, phenolic glycolipid (PGL-1), can be detected. We purified PGL-1 from the infected tissues of armadillos with Mycobacterium leprae, and ascertained the preparations by NMR. In vivo injection of the PGL-1 in a micell form into mice induced remarkable suppression in cell-mediated immunity: The inhibition of Con A-induced T cell proliferation and the inhibition of generation of allogenic killer T cells. Addition of the PGL-1 on the normal splenic cultures also resulted the suppression of Con A responses and generation of allogenic killer T cells. These suppression by PGL-1 were completely abrogated by the supplementaion with normal peritoneal macrophages, suggesting that the inhibition by PGL-1 was due to the injuries in macrophages.
We also isolated a mycolic acid-containing glycolipid (GaGM) derived from Gordona aurantiaca, an acid-fast bacterium closely related with Mycobacterium. This glycolipid had simillar effects on the immunological responses in a micell form. However, liposomes containing GaGM enhanced generation of cytotoxic t cells, NK cells, and activity of antibody dependent cytotoxicity (ADCC). These results suggest that effects of gycolipids were affected by their forms for presentaion to the host.
|
