| Project/Area Number |
62570203
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Virology
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| Research Institution | Osaka University |
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Principal Investigator |
IKUTA Kazuyoshi Res. Inst. Microbial Dis., Osaka Univ., Associated Professor, 微生物病研究, 助教授 (60127181)
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| Project Period (FY) |
1987 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1988: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1987: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Marek's disease virus / herpesvirus / oncogenic virus / in vitro transformation / interleukin 2 / 可移植性 / 腫瘍関連抗原 / トランスフォーム遺伝子 / Tリンパ芽球様細胞 |
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| Research Abstract |
1). Continuously proliferating T-lymphoblastoid cells, named MDCC-MTBl, were obtained by infection of chick embryo lymphocytes with marek's disease virus serotype 1 (MDVl), in cluture and subsequent cultivation in the presence of interleukin 2. This cell line was highly transplantable in chicks, and formed solid lymphomas in various organs. Proliferating cells derived from the transplanted MTBl cells were obtained on culture of the spleen cells of this chick. When the MTBl cells were examined for P antigen expression by immunofluorescence test with the monoclonal antibody against MDVl-specific phosphorylated polypeptides, which seems to be T antigen, it was found that the percentage of P antigen-positive cells after the treatment with IUdR was significantly increased by the transplantation of the cells into chicks.
2). We identified oncogenic MDVl-specific DNA structures (BamHI-D and -H). These structures were also identified in MTBl cells. Northern blot hybridization revealed that the major poly(A) mRNA of 2.3 kb and the less abundant one of 4.6 kb were encoded from these regions.
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