ROLE OF CYTOSKELETON AND INTERNAL MEMBRANES ON PICONRAVIRUS RNA REPLICATION

Research Project

Project/Area Number	62570208
Research Category	Grant-in-Aid for Scientific Research (C).
Allocation Type	Single-year Grants
Research Institution	NATIONAL INSTITUTE OF HEALTH
Principal Investigator	TAKEDA NAOKAZU NATIONAL INSTITUTE OF HEALTH, ウィルス中央検査部, 主任研究官 (90132894)
Project Period (FY)	1987
Project Status	Completed (Fiscal Year 1987)
Keywords	Enterovirus 70 / Picornavirus RNA replication / In vitro RNA systhesis / Protein primer / 蛋白のウリジル化 / エンテロウイルス70 / ピコルナウイルスRNA複製 / in vitro RNA合成 / 蛋白のラリジル化
Research Abstract	AN IN VITRO ENTEROVIRUS 70 RNA SYNTHESEZING SYSTEM DERIVED FROM A CRUDE MEMBRANE FRACTION OF INFECTED HeLa CELLS WAS USED TO ANALYZE THE MECHANISM OF OF ENTEROVIRUS 70 PLUS-STRAND RNA SYNTHESIS. THIS SYSTEM IS CAPABLE OF SYNTHESIZING THE NUCLEOTIDYL-PROTEINS, VPg-pU AND VPg-pUpU, THE 5'-TERMINAL STRUCTURE OF PLUS-STRAND RNA. ELONGATION REACTION OF THE NUCLEOTIDYL PROTEINS WAS STIMULATED BY THE ADDITION OF A SOLUBLE FRACTION (S-10) OBTAINED FROM UNINFECTED HeLa CELLS, INDICATING THAT THIS REACTION MAY BE STIMULATED BY A HOST CELLULAR FACTOR (S).