| Project/Area Number |
62570260
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Legal medicine
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| Research Institution | University of Tsukuba |
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Principal Investigator |
MISAWA Shogo Institute of Community Medicine, University of Tsukuba, Professor, 社会医学系, 教授 (50086534)
三沢 章吾 (1987) 筑波大学, 社会医学系, 教授
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| Co-Investigator(Kenkyū-buntansha) |
TANAKA Einosuke Institute of Community Medicine, University of Tsukuba, Lecturer, 社会医学系, 講師 (30138416)
TSUCHIYA Shigeru Institute of Community Medicine, University of Tsukuba, Professor, 社会医学系, 教授 (10013963)
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| Project Period (FY) |
1987 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1988: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1987: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Postmortem interval / Third component of complement (C3) / Complement cleavage / Crossed immunoelectrophoresis / _1C / B_1C / 補体C3 / 補体第3成分分解 / 交差免疫電気泳動法 |
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| Research Abstract |
To estimate postmortem interval(PMI), spontaneous cleavage of the third component of complement (C3) was studied in aged blood and cadaveric blood by crossed immunoelectrophoresis. C3 cleavages in vitro at different temperatures showed that the incubation of whole blood at a higher temperature led to a faster conversion of beta IC (native C3) to beta 1A (C3_c). In cadaveric blood, we found a significant positive correlation between percentage of C3 cleavage and pmi. From these results, it is possible to estimate PMI from the ratios of C3 cleavege. Using the kinetics of C3 cleavage in vitro described as dC/dt = -kC, where C is the consentration of native C3 at time t and k is a first-order rate constant, Arrhenius' equation, and another equation which assumes a linear drop of body temperature after death, the percentages of C3 cleavage were calculated. There was a significant positive correlation between the calculated percentages and the measured percentages of up to 10% in cadaveric blood. We found that the comparison between the calculated percentage of C3 cleavage for each optional postmortem interval and the measured percentage of up to 10% in cadaveric blood leads to the estimation of PMI. This approach is one step towards the development of an accurate method for determining PMI based on C3 cleavage, that is, on a first-order reaction.
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