| Project/Area Number |
62570261
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Legal medicine
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| Research Institution | GUNMA UNIVERSITY |
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Principal Investigator |
YAZAWA Shin Dept. Legal Med., School of Med., Gunma University., 医学部, 講師 (10008386)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAJIMA Tamiko Dept. Legal Med., School of Med., Gunma University., 医学部, 助手 (40008561)
FURUKAWA Ken Dept. Legal Med., School of Med., Gunma University., 医学部, 教授 (90008227)
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| Project Period (FY) |
1987 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1988: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1987: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Blood group antigens / Gene encoded fucosyltransferases / Genetic biochemistry / Chemically synthesized oligosaccharides / フコシルトランスフェラーゼ / 化学合成オリゴ糖 / 霊長類の血液型 / 血液型糖鎖抗原の遺伝生化学 / ルイス抗原(a.b.X.Y) / α-フコシルトランスフェラーゼ / 抗ルイスモノクローナル抗体 / ラジオイムノアッセイ / 血液型交換 / 腫瘍関連抗原 |
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| Research Abstract |
Detection and characterization of -fucosyltransferases responsible for the synthesis of fucoglycoconjugates in blood group substances were undertaken to elucidate the relationship between -fucosyltransferases and fucosyl blood group substances which were primary and secondary products of genes such as H, Se, Le and X.
1) The availability of new chemically synthesized oligosaccharides for determining the specificities of glycosyltransferases could provide specific assay systems for (1 2)-, (1 3)-, (1 4)-L-fucosyltransferases, N-acetyl-D-glucosaminyltransferase and sialyltransferases. 2) A simple radioimmunoassy method was developed for the measurement of Lewis antigens in human sera and saliva with monoclonal anti-Le^a and anti-Le^b antibodies. Using this immunoassay method, the expression of incompatible lewis blood group antigens was demonstrated in new-born, pregnant samples and samples from cancer patients. (1 4)-L-Fucosyltransferase responsible for Le^a antigen
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