| Project/Area Number |
62570285
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Legal medicine
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| Research Institution | Kyoto University |
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Principal Investigator |
KUMAGAI Shunichi Faculty of Medicine, Kyoto University, 医学部, 助手 (00153346)
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| Co-Investigator(Kenkyū-buntansha) |
YODOI Junji Faculty of Medicine, Kyoto University, 医学部, 助手 (80108993)
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| Project Period (FY) |
1987 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1988: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1987: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | Systemic Lupus Erythematosus / Interleukin / Interleukin-2 / Interleukin-2 receptor / 強皮症 / IL-2 / IL-2レセプター / T細胞 |
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| Research Abstract |
The expression of interleukin-2 receptor (IL-2R) in patients with systemic lupus erythematosus (SLE) was investigated in order to clarify the causes of impaired functions of their T cells.
1. Studies for IL-2 production of peripheral lymphocytes.
The IL-2 production of PHA-stimulated lymphocytes was significantly decreased in patients with SLE. However, PSS patients showed markedly enhanced IL-2 production, which was derived from hyperactivity of thier CD4-positive T cells.
2. Studies for expression of IL-2R on PHA-activated peripheral lymphocytes in patients with SLE.
(1) When the expression of IL-2R/p55 was examined using FITC-conjugated or ^<125>-I labeled anti-Tac monoclonal antibody, both the percentage of Tac-positive T cells and the number of Tac epitope were not different between SLE patients and normal subjects.
(2) The IL-2R expression of PHA-activated lymphocytes from SLE patients was further studied by the scatchard plot analysis using ^<125>-labeled recombinant IL-2. The prominent defect in expression of high-affinity IL-2R was demonstrated in patients with SLE.
3. Using FITC-conjugated IL-2, we have established the new and easy method which mainly detected the high-affinity IL-2R. By use of this mithod, the defective expression of high affinty IL-2R was clearly shown on activated t cells of SLE patients in spite of thier normal expression of Tac antigen. The defect was more prominent on thier CD4-positive T cells.
In summary, T cells of SLE patients was shown to have a defect in expression or regulation of high-affinity IL-2R, which may cause various dysfunctions in the patients.
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