| Project/Area Number |
62570307
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Gastroenterology
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| Research Institution | University of Tsukuba |
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Principal Investigator |
KOYAMA Shohei Dept. Internal Med., Inst. Clin. Med., Univ. of Tsukuba., 臨床医学系, 講師 (00110502)
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| Project Period (FY) |
1987 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1988: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1987: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | malignant ascites / lymphokine-activated killer (LAK) cells / Lymphokine-activated suppressor (LAS) cells / recombinant interleukin 2 (rIL 2) / T-cell growth factor (TCGF) / killer T cells / monoclonal antibody / 免疫応答 / ヘンターロイキン2(IL2) / T細胞増殖因子(TCGF)モノクローナル抗体 / Two-color flow cytometry / TCGF / r1L2 / Two-colon flow ceytometry |
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| Research Abstract |
1. Analysis of tumor-associated antigens. Monoclonal antibodies against human gallbladder and pancreatic carcinomas were produced by our laboratories. Immunochemical and immunohistological analysis of tumor-associated antigens defined by these monoclonal antibodies were performed. 2. Relationships between peripheral blood lymphocytes and tumor cells. Differential activation of lymphokine-activated killer (LAK) cells with different surface phenotype by cultivation with recombinant interleukin 2 (rIL 2) or T-cell growth factor (TCGF) in gastric cancer patients. However, TCGF generated a lymphokine-activated suppressor (LAS) cells in PBL from patients with systemic metastasis of gastric carcinoma. 3. Relationships between spleen cells and tumor cells. RIL 2-activated spleen cells from cancer pataients revealed a strong LAK cells activities with surface phenotype of CD8^+CD11^- and leu 7^+CD16^- cells. This data indicated that spleen cells differentiated into LAK cells with different surface phenotype as compared with that of rIL 2-activated PBL. LAS cells were not generated from spleen cells in cancer patients. 4. Relationships between lymphoid cells in malignant ascites and tumor cells. TCGF-activated lymphoid cells from malignant ascites revealed a strong cytolytic activities against autologous and/or allogeneic tumor cells. This system induced autologous mixed lymphocytetumor reaction and autologous mixed lymphocytes reaction. Thus specific and non-specific killer T cells with surphase marker, CD8^+CD11^- capable of lysing autologous tumor were generated. Adaptive immunotherapy may become applicable for clinical therapy by using the killer T cells proliferated by TCGF.
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