Study of cytotoxic Killer T cell against target liver cells and effect of prostaglandins - inhibitory effect of prostaglandin El on T cell-mediated cytotoxicity.
Project/Area Number |
62570308
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Research Category |
Grant-in-Aid for General Scientific Research (C)
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Allocation Type | Single-year Grants |
Research Field |
Gastroenterology
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Research Institution | Chiba University School of Medicine |
Principal Investigator |
MORI Yoshio The First Department of Med. Chiba Univ. School of Med., 医学部内科学第一, 文部教官助手 (40174388)
|
Co-Investigator(Kenkyū-buntansha) |
WAKASHIN Masafumi The First Department of Medicine, Chiba Univ. School of Med., 医学部内科学第一, 文部教官講師 (60009595)
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Project Period (FY) |
1987 – 1988
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Project Status |
Completed (Fiscal Year 1988)
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Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1988: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1987: ¥2,000,000 (Direct Cost: ¥2,000,000)
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Keywords | Experimental hepatitis / Cytotoxic killer T cell / Prostaglandin El / インドメサシン / 肝特異的キラーT細胞 / 細胞障害性キラーT細胞(CTL) / 肝障害 / プロスタグランディン / PGE_1 |
Research Abstract |
The effects of prostaglandin El on cell-mediated cytotoxicity against hepatocytes were investigated using an in vitro cytotoxic assay system. Isolated liver cells from normal C57BL/6 mice were used as the target cells, and effector cells were obtained from spleen cells of C57BL/6 mice in which experimental hepatitis had been induced by immunization with syngeneic liver antigens. In this assay system, spleen T cells adherent to nylon wool demonstrated a high cytotoxicity against liver cells. The cytotoxicity was markedly reduced by prostaglandin El at concentrations 10^7 M. Maximum suppressive activity was obtained when prostaglandin El was continuously present during the assay period. By contrast, indomethacin, a specific inhibitor of prostaglandin sythesis, enhanced the cytotoxic activity of effector cells. These data seem to indicate that exogenously added prostaglandin El has an inhibitory effect on cell-mediated cytotoxicity of effector spleen cells against target hepatocytes.
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Report
(3 results)
Research Products
(26 results)