Clinical and Experimental Study of Production of Abnormal Prothrombin PIVKA-II in Hepatocellular Carcinoma.
| Project/Area Number |
62570337
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Gastroenterology
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| Research Institution | Tokyo Women's Medical College |
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Principal Investigator |
OKUDA Hiroaki Division of Medicine,Institute of Gastroenterology,Tokyo Women's Medical College, 消化器内科, 助手 (60119922)
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| Project Period (FY) |
1987 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1988: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1987: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Hepatocellular carcinoma / PIVKA-II / Vit. K / Hepatoma cell / Prothrombin related antigen level / F-II / vit.K / プロトロンビン前駆体 / プロトロンビン関連抗原 |
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| Research Abstract |
Fifty-nine percent of patients with hepatocellular carcinoma (HCC) had elevated plasma PIVKA-II levels. None of the cirrhotic patients had elevated pivka-ii levels. Thus PiVKA-II has a high specificity for HCC. There was no correlation between PICKA-II and AFP levels indicating that the combination of these 2 markers seems to be useful in the diagnosis of HCC. There was a correlation between tumor size and PIVKA-II levels in HCC. The half-life of PIVKA-II in HCC patients was 60 hr and was identical to that in vit. K deficiency patients, suggesting that PIVKA-II in HCC patients has the same vit. K sensitivity as that in vit. K deficiency patients. Thus it seems useful to follow HCC patients with PIVKA-II measurement after therapy for its half-life and reduction rate. PIVKA-II was detected in the cultures of 5 hepatoma cell lines and was not detected in non-hepatoma cell lines. There was a marked decrease of PIVKA-II with added vit.K.Thus PIVKA-II is produced by hepatoma cells and its production is dependent on the amount of vit. K available. Using hepatoma cell line huH-2, we found out that all 3 enzymes involved in the production of normal prothrombin, K-epoxide-reductase, K-reductase (vit. K cycle) and -glutamyl-carboxylase, functions in huH-2. The cancerous tissue of HCC patients positive for plasma PIVKA-II showed high PIVKA-II levels and the non-cancreous tissur showed low levels. Prothrombin related antigen levels F-II was higher in cancerous tissue than in non-cancerous tissue. The cancerous tissue of PIVKA-II positive HCC patients showed a more marked F-II staining than in PIVKA-II negative HCC patients. There was postitve PIVKA-II staining in the cancerous tissue of several HCC patients. Thus production of either PIVKA-II or F-II was suggested in hepatoma cell. Is was suggested that increased formation of F-II occurs in HCC, resulting in relative vit. K deficiency and appearance of PIVKA-II.
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Report
(3 results)
Research Products
(23 results)
