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Airway-hyperreactivity and arachidonic acid metabolism induced by various stimulations.

Research Project

Project/Area Number 62570354
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Respiratory organ internal medicine
Research InstitutionNational Institute for Environmental Studies

Principal Investigator

KOBAYASHI Takahiro  NIES, Basic Medical Sciences, Senior research scientist, 環境生理部・慢性毒性研究室, 主任研究員 (50124342)

Co-Investigator(Kenkyū-buntansha) YAMANE Kazusuke  NIES, Basic Medical Sciences, Scientist, 環境生理部・慢性毒性研究室, 研究員 (40182589)
Project Period (FY) 1987 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1989: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1988: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1987: ¥900,000 (Direct Cost: ¥900,000)
Keywordsairway hyperreactivity / tracheal smooth muscle / guinea pig / airway reactivity / arachidonic acid metabolites / chemical mediator / analogue of thromboxane / 硫酸エアロゾル / U46619 / プロスタグランジンE_2 / 電場刺激 / オゾン / 二酸化窒素 / トロンボキサンA_2 / OKY-046 / ヒスタミン / ロイコトリエンC_4 / イソプロテレノール / アナフィラキシー反応 / シクロオキシゲナーゼ / リポキシゲナーゼ / プロスタグランジン / ロイコトリエン
Research Abstract

In order to elucidate a mechanism of induction of airway hyperreactivity, it is important to clarify the relationship between arachidonic acid (AA) metabolites and airway hyperreactivity. Therefore we investigated on an effect of AA metabolites on reactivity of tracheal smooth muscle to chemical mediators, how to induce the airway hyperreactivity and analysis of a mechanism of airway hyperreactivity. Comparison of the reactivity and sensitivity of the tracheal smooth muscle to histamine in the presence and absence of endogenously released AA metabolites revealed that endogenous AA metabolites do not affect on the reactivity and sensitivity. Exogenously added leukotriene C_4, ananalogue of thromboxane A_2 or pros taglandin E2, did not affect on the reactivity and sensitivity of the tracheal smooth muscle.Exposure of O_3, NO_2 or H_2SO_4 aerosol induce airway hyperreactivity in guinea pigs. The NO_2 induced airway hyperreactivity was abolished by administration of OKY 046, an inhibitor of thromboxane synthetase. NO_2 and O_3 exposure inhibitprostacyclin synthetase markedly. At the time of induction of airway hyperreactivity, however, prostacyclin synthetase was notinhibited. Anaphylactic reaction also induces airway hyperreactivity. Comparison of the reactivity and sensitivity of the tracheal smooth muscle to histamine pre and post anaphylactic reaction revealed that anaphylactic reaction in vivo do not affect the reactivity and sensitivity to histamine, LTC_<C4> and isoproterenol in tracheal smooth muscle in vitro. On the contrary, reactivity to U46619 increased significantly. In conclusion, endogenously and exogenously added AA metabolites did not affect on the reactivity and sensitivity of the trachealsmooth muscle. Thromboxane A_2 could be one of an important factor of airway hyperreactivity induced by NO_2 exposure in vivo and hyperreactivity of tracheal smooth muscle induced by anaphylactic reaction in vivo.

Report

(4 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • 1987 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] K.Yamane,T.Kobayashi: "Endogenous AA metabolites and their possible role in tracheal smooth muscle tone in guinea pig." J.Appl.Physiol.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 山根一祐,小林隆弘: "オゾン暴露がモルモット気管平滑筋の自発性収縮ならびにヒスタミンに対する反応性に及ぼす影響" 国立公害研究所報告. 115. 197-203 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] T.Kobayashi,Y.Shinozaki: "Sub-acute exposure to nitrogen dioxide induces" Amer Rev.Respir Disease.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 小林隆弘,三浦卓: "SPMについて「地域における大気汚染による健康被害の予防システムのあり方に関する研究」" 大気汚染研究協会報告. 64-76 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] K.Yamane, T.Kobayashi: "Endogenous AA metabolites and their possible role in tracheal smooth muscle tone in guinea pig." J.Appl.Physiol (1990).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] K.Yamane, T.Kobayashi: "Effect of ozone exposure on the inherent tone and on the responsibility to histamine in guinea pig tracheal smooth muscle" Research Report from National Institute for Environmental Studies, Japan, 119, 197-204 (1988).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] T.Kobayashi, Y.Shinozaki: "Sub-acute exposure to nitrogen dioxide induces airway hyperreactivity in guinea pigs." Amer.Rev.Respir. Disease 1990.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] T.Kobayashi, T.Miura: "Effect of suspended particulate matter." Research Report from Japan society of Air Pollution pp.64-76 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] K.Yamane,T.Kobayashi: "Endogenous AA metabolites and their possible role in tracheal smooth tone in guinea pig." J.Appl.Physiol.

    • Related Report
      1989 Annual Research Report
  • [Publications] 山根一祐,小林隆弘: "オゾン暴露がモルモット気管平滑筋の自発性収縮ならびにヒスタミンに対する反応性に及ぼす影響" 国立公害研究所報告. 115. 197-203 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] T.Kobayashi,Y.Shinozaki: "Subーacute exposure to mitrogen dioxide induces" Amer Rev Respir Disease.

    • Related Report
      1989 Annual Research Report
  • [Publications] 小林隆弘,三浦卓: "SPMについて「地域における大気汚染による健康被害の予防システムのあり方に関する研究」" 大気汚染研究協会報告. 64-76 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 山根一祐、小林隆弘: J.Appl.Physiol.

    • Related Report
      1988 Annual Research Report
  • [Publications] 山根一祐、小林隆弘: 国立公害研究所報告. 197-203 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] K.Yamane;T.Kobayashi: Jop.J.Pharmacol.49. 166 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Kazusuke Yamane & Takahiro Kobayashi: J. Appl. Physiol.

    • Related Report
      1987 Annual Research Report
  • [Publications] 山根一祐,小林隆弘: 国立公害研究所研究報告.

    • Related Report
      1987 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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