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Effects of IL-3 and IL-4 on proliferation and transdifferentiation of mast cells

Research Project

Project/Area Number 62570419
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Pediatrics
Research InstitutionShinshu University

Principal Investigator

NAKAHATA Tatsutoshi  Department of Pediatrics, Shinshu University, School of Medicine, 医学部附属病院, 講師 (20110744)

Co-Investigator(Kenkyū-buntansha) NAGANUMA Kuniaki  Department of Pediatrics, Shinshu University, School of Medicine, 医学部, 助手 (90180504)
Project Period (FY) 1987 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1988: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1987: ¥1,700,000 (Direct Cost: ¥1,700,000)
Keywordsconnective tissue-type mast cells(CTMC) / mucosal mast cells(MMC) / methycellulose culture / interleukin 3(IL3) / interleukin 4(IL-4) / TPA / メチルセルロース培養 / インターロイキン3(IL-3) / インターロイキン4(IL-4) / CFU-Mast / IL3 / IL4 / シグナル伝達機構 / プロテインキナーゼC
Research Abstract

There are two phenotypically distinct subpopulatins of mast cells : connective tissue-type mast cells (CTMC) and mucosal mast cells(MMC). These populations differ in their location, cell sizu, staining characteristics, mediator content and factor dependency. Although proliferation of MMC was known to be T cell dependent in vivo and thought to be interleukin 3(IL-3) dependent in vitro, the factors on which CTMC proliferation depends remained elusive. We recently found that mature CTMC separated from mouse peritoneal cells could proliferate extensively in methylcellulose culture and maintain the appearance and function of CTMC (Nature 1986).
In this study, we have indicated that clonal growth of CTMC could not be induced by IL-3 alone and required the presence of both IL-3 and interleukin 4 (IL-4). We also tested whether IL-3 can act co-stimulator for CTMC proliferation in the compinations with various phorbol derivatives. Neither IL-3 nor 12-0-tetradecanoylphorbol-13 acetate(TPA) stimulated mast cell colony growth from BDF_1 mice peritoneal CTMC purified by percoll density-gradient centrifugation. However, when IL-3 and TPA were supplemented simultaneously in the culture, numerous CTMC-type mast cell colonies developed. Other 3 phorbol derivatives, which are capable of activating protein kinase C (PKC), also induced proliferation of CTMC in the presence of IL-3, but other 3 phorbol derivatives, all of which are unable to activate PKC, failed to induce the coliny growth. Continuous presence of IL-3 and TPA in culture was required for extensive proliferation of CTMC. These results indicate that activation of PKC may act inportant role to induce the proliferation of CTMC in vitro. Cytological examination of individual colonies suported by IL-3 and IL-4 suggestthe transdifferentiation from mature CTMC to alcian blue(+)-safranin(-)-Berberine(-) MMC like cells in vitro.

Report

(3 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • Research Products

    (38 results)

All Other

All Publications (38 results)

  • [Publications] Nakano Toru: Fed.Proc.46. 1920-1923 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Okai Yasuji: Immunol.Letters. 17. 1-5 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Koike Kenichi: J.Exp.Med.168. 879-890 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kobayashi Toshimi: J.Cell.Physiol.138. 24-28 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Okano Akira: Transplantation.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tsuji Kohichiro: J.Cell.Physiol.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yoshizaki Kazuyuki: New Engl.J.Med.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 中畑龍俊: 小児内科. 19. 1067-1074 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 中畑龍俊: 15. 275-281 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 中畑龍俊: 2. 124-128 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 中畑龍俊: 医学のあゆみ. 146. 273-277 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 中畑龍俊: "白血病細胞の生物医学的特性とその制御" 癌と科学療法社, 57-67 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 中畑龍俊: "小児科学年鑑1988" 診断と治療社, 124-128 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 中畑龍俊: "Annual Review 血液 1989" 中外医学社, 248-259 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Toru Nakano: "Genetically mast-cell deficient W/W^V mice as a tool for studies of differentiation and function of mast cells." Fed. Proc.46. 1920-1923 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yasuji Okai: "Identification of colony-forming activities of various hemopoietic cells in the serum-free culture medium of a human embyo fibroblast cell strain." Immunol. Letters. 17. 1-5 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kenichi Koike: "Synergism of BSF-2/interleukin 6 and interleukin 3 on development of multi-potential hemopoietic progenitors in serum-free culture." J. Exp. Med.168. 879-890 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Toshimi Kobayashi: "A stochastic model for mast cell proliferation in culture of murine peritoneal cells." J. Cell. Physiol.138. 24-28 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Akira Okano: "Accerelated recovery of hematopoiesis in bone marrow transplanted mice by human recombinant interleukin-6/B cell stimulatory factor-2(IL-6/BSF-2)." Transplantation. in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kohichiro Tsuji: "A stochastic model for multipotent hemopoietic progenitor differentiation." J. Cell. Physiol.in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kazuyuki Yoshizaki: "Pathologic significance of interleukin 6 (IL-6/BSF-2) in Castleman's disease." New Engl. J. Med.in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Okai Yasuji: Immunol.Letters. 17. 1-5 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Koike Kenichi: J.Exp.Med.168. 879-890 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Kobayashi Toshimi: J.Cell.Physiol.138. 24-28 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Tsuji Kohichiro: J.Cell.Physiol.in press.

    • Related Report
      1988 Annual Research Report
  • [Publications] Yoshizaki Kazuyuki: New Engl.J.Med.in press.

    • Related Report
      1988 Annual Research Report
  • [Publications] Okano Akira: Transplantation. in press.

    • Related Report
      1988 Annual Research Report
  • [Publications] 中畑龍俊: 15. 275-281 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 中畑龍俊: 2. 124-128 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 中畑龍俊: 医学のあゆみ. 146. 273-277 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 中畑龍俊: "小児科学年鑑1988" 診断と治療社, 124-128 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 中畑龍俊: "Annual Review 血液" 中外医学社, 248-259 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 中畑龍俊: Medical Immunology. 15. 275-281 (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] 安藤伯秋: 日本血液学会雑誌. 50. 1290-1296 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 中畑龍俊: 小児内科. 19. 1067-1074 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Nakano T: Federation Proceedings. 46. 1920-1923 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Okai Yasuji: Immunology Letters. 17. 1-5 (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] 中畑龍俊: "白血病細胞の生物医学的特性とその制御(分担;肥満細胞, 好塩基球の増殖と分化)" 癌と化学療法社, 453(57-67) (1987)

    • Related Report
      1987 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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