Neurochemical and histochemical studies on the mechanism and prevention of cerebellar under-development due to perinatal hyperbilirubinemia
| Project/Area Number |
62570446
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pediatrics
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| Research Institution | Institute for Developmental Research |
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Principal Investigator |
KEINO Hiroomi Department of Perinatology.Chief of Section, 周生期学部, 室長 (30090426)
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| Co-Investigator(Kenkyū-buntansha) |
NAGAE Hidetoshi Toyata Memorial Hospital.Medical doctor, 小児科, 医師
AONO Sachiko Department of Perinatology.Assistant of experiment, 周生期学部, 助手 (20231780)
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| Project Period (FY) |
1987 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1988: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1987: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | neonatal-jaundice / bilirubin / cerebellar hypoplasia / drugtherapy / hemeoxygenase / tin-protoporphyrin / 動物モデル / ヘムオキシゲナーゼ / 錫プロトポルフィリン / プルキンエ細胞 |
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| Research Abstract |
It is known that the cerebellar hypoplasia of jaundiced homozygous (j/j) Gunn rats is caused by neonatal hyperbilirubinemia. The hypoplasia is more severely exhibited in the antero-medial lobules and even less in the posterior lobules. The histochemical observations showed that Purkinje cells in the antero-medial lobules were more acidic than the posterior, and that a larger quantity of bilirubin was bound to purkinje cells in the former which is sensitive to bilirubin than in the latter which is tolerant to the pigment.
Tin-protoporphyrin (SnPP) is a powerful competitive inhibitor of the heme oxygenase and is now tested clinically for jaundiced patient. Intraperitoneal or subcutaneous administration of SnPP to j/j rat neonates caused a rapid decrease of the serum bilirubin level, an apparent reduction of the mortality rate and hence protection against the cerebellar hypoplasia. Unexpectedly, however, the cerebellar underdevelopment of j/j rats was not so markedly improved. The combination of SnPP treatment and photoirradiation induced death with a high mortality; more than half of the infants were dead 4h after treatment by 20
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Report
(3 results)
Research Products
(17 results)
