• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Epidermal IL-1 Function and Skin Disease

Research Project

Project/Area Number 62570464
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Dermatology
Research InstitutionTeikyo University

Principal Investigator

MIZOGUCHI Masako  Dept. of Derm. Teikyo Univ. School of Medicine, 医学部皮膚科, 教授 (30010250)

Co-Investigator(Kenkyū-buntansha) KAWA Yoko  Dept. of Derm. Teikyo Univ. School of Medicine, 医学部皮膚科, 研究助手 (10082273)
FURUSAWA Shuichi  Dept. of Derm. Teikyo Univ. School of Medicine, 医学部皮膚科, 助手 (80130037)
Project Period (FY) 1987 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1988: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1987: ¥1,100,000 (Direct Cost: ¥1,100,000)
Keywordsinterleukin 1,ETAF / 接触性皮膚炎 / 基底細胞上皮腫 / 有棘細胞癌 / interleukin 1 / ETAF / in situハイブリダーゼーション
Research Abstract

1) Interleukin 1 Production by Epidermal Cells in Contact Hypersensitivity (CHS). Using C3H/HeN mice, we studied the levels of epidermal IL-1 activity and mRNA for IL-1 alpha and beta during CHS reaction. Epidermis with dDNFBinduced CHS and UVB-durned epidermis, were examined. The lysates of the epidermis were used to estimate IL-1 activity, which was measured by C3H/HeJ mouse thymocyte proliferation assay. The mRNA levels for epidermal IL-1 alpha and bata were estimated by cytoplasmic dot hybridization. Both the IL-1 activity and the mRNA levels for IL-1 alpha and beta were increased at 6 HRS after UVB irradiation. However, concerning chs reaction at 6-24 HRS after challenge, the IL-1 activity decreased but IL-1 mRNA levels did not change. We performed mixed cell cultures using DNP-modified epidermal cells and primed t cells from lymphonodi from sensitized mice. The proliferation of primed t cells was detected, but IL-1 mRNA levels in the mixed culture did not change. These data sugge … More st that futher production of epidermal IL-1 may not occur during CHS reaction.
2) Interleukin 1 Alpha and Beta in Squamous Cell Carcinoma (SCC) and Basal Cell Epithelioma (BCE). The location of eqidermal IL-1 in notmal human skin, SCC, and BCE were examined by an immunohistological technique, using polyclonal antibodies against HRIL-1 alpha and beta. The staining intensity with the anti-IL-1 alpha was higher than that with the anti-IL-1 beta. The staining intensity was increased in SCC, but decreased in BCE. IL-1 alpha and beta activity of the lysate was examined by neutralizing ability of anti-IL-1 alpha or beta on thymocyte proliferation assay. Only IL-1 alpha activity was detected from these tumors, however, the activity of SCC was higher than that of BCE. By northern hybridization, the mRNA expression for IL-1 alpha was strongly detected in the two tumors, but that for IL-1 beta was slightly detected, suggesting that both SCC and BCE cells have mRNA for IL-1 alpha and beta. IL-1 beta activity may not have been detected because of IL-1 beta inhibitor or unknown factors. Less

Report

(3 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] 呉貴郷: 帝京医学雑誌. 11. 361-369 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 溝口昌子: "現代皮膚科学大系追補 第1巻 炎症化学伝達物質、インターロイキン1など" 中山書店, 10 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Masako Mizoguchi: "Macrophage-derived interleukin 1 activity in atopic dermatitis. (abstract)" J Invest Dermatol. 84. 303 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kikyo Goh: "Polymorphonuclear leukocytes-derived IL-1." Proc Jap Soc Invest Dermatol. 12. 46-47 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Shuichi Furusawa: "Production of IL-1 alpha and beta by human peripheral polymorphonuclear neutrophils." J Invest Dermatol. 92. 431 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Masako Mizoguchi: "Interleukin 1 Alpha and Beta in Squamous Cell Carcinoma and Basal Cell Epithelioma" J Invest Dermatol. 92. 483 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 呉貴郷: 帝京医学雑誌. 11. 361-369 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Kikyo Goh.: Proc Jap Soc Invest Dermatol. 12. 46-47 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Kikyo Goh.: Int Arch Allergy Appl Immunol. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Shuichi Furusawa.: J Invest Dermatol. 92. 431 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Masako Mizoguchi.: J Invest Dermatol. 92. 483 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] M.Mizoguchi,S.Furusawa,S.Okitsu, & K.Yoshino: J.Invest Dermatol.84. 303 (1985)

    • Related Report
      1987 Annual Research Report
  • [Publications] 呉 貴卿,河 陽子,古澤修一,溝口昌子: Proc.Jap.Soc.Invest.Dermatol.12. (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] K.Goh,S.Furusawa,Y.Kawa,S.Okitsu & M.Mizoguchi.: J.Immunology.

    • Related Report
      1987 Annual Research Report
  • [Publications] 溝口 昌子: "現代皮膚科学大系追補第1巻 炎症化学伝達物質,インターロイキン1など" 中山書店, 124-133 (1987)

    • Related Report
      1987 Annual Research Report

URL: 

Published: 1987-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi