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Alteration of glucose transporter in diabetic state.

Research Project

Project/Area Number 62570504
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 内分泌・代謝学
Research InstitutionUniversity of Tokyo

Principal Investigator

OKA Yoshitomo  Faculty of Medicine, University of Tokyo, 医学部(病), 助手 (70175256)

Co-Investigator(Kenkyū-buntansha) ASANO Tomoichiro  Faculty of Medicine, University of Tokyo, 医学部(病), 医員
SHIBASAKI Yoshikazu  Faculty of Medicine, University of Tokyo, 医学部(病), 助手 (80196419)
KASUGA Masato  Faculty of Medicine, University of Tokyo, 医学部(病), 助手 (50161047)
Project Period (FY) 1987 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1988: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1987: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsGlucose transporter / Glucose transporter mRNA / 糖尿病 / 心臓
Research Abstract

We raised three antipeptide antibodies against three different domains of HepG2 type glucose transporter. These antibodies recognized only 3 and 30 % of all the glucose transporters presnt in rat adipocyte and brain, respectively, suggesting the presence of more that two types of glucose transporter in rat adipocyte and brain. Western blotting of the brain membranes from streptozotocin-induced diabetic rats with these antibodies showed no difference in the glucose transporter amount, compared with normal rats or insulin-treated diabetic rats. In addition, photoaffinity-labeling of glucose transporter with [^3H] cytochalasin B showed no alteration of glucose transporter amount in the brain from diabetic rats. Furthermore, no significant changes in the amount of HepG2 type glucose transporter mRNA was observed in the brain from diabetic rats when Northern blotting was performed with rabbit brain HepG2 type glucose transporter cDNA as a probe. The similar results were observed in the heart from diabetic rats, i. e. no significant changes were observed in the amount of both glucose transporter protein and HepG2 type glucose transporter mRNA compared with normal rats or insulin-treated diabetic rats.
These results showed a remarkable contrast to the results observed in the adipocytes from diabetic rats, indicating that different mechanisms regulate the amount of glucose transporter in different tissues in diabetic state.

Report

(3 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] Yoshitomo Oka.: The Journal of Biological Chemistry. 263. 13432-13439 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yoshitomo Oka: "Studies with antipeptide antibody suggest the presence of at least of two types of glucose transporter in rat brain and adipocyte" The journal of Biological Chemistry. 263. 13432-13439 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yoshitomo,Oka.: The Journal of Biological Chemistry. 263. 13432-13439 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Y.OKA, T.ASANO, M.KASUGA, Y.KANAZAWA, F.TAKAKU: Diabetes. 36(SUPPL1). 83A (1987)

    • Related Report
      1987 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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