Effect of recombinant colony stimulating factors on the proliferation and differentiation of blast progenitors in acute myeloblastic leukemia.
| Project/Area Number |
62570554
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Hematology
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| Research Institution | Tokyo Women's Medical College |
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Principal Investigator |
MOTOJI Toshiko Tokyo Women's Medical College Instructor, 医学部, 講師 (00101808)
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| Project Period (FY) |
1987 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1988: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1987: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Leukemic colony formation / acute myeloblastic leukemia / recombinant granulocyte-macrophage colony stimulating factors / recombinant granulocyte colony stimulating factors / 遺伝子組み換え型顆粒球コロニー形成刺激因子 / 遺伝子組み換え型インターロイキン3 / 他のコロニー形成刺激因子との併用作用 / ヒト遺伝子組み換え型顆粒球単球コロニー形成刺激因子 / ヒト遺伝子組み換え型顆粒球コロニー形成刺激因子 |
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| Research Abstract |
The colony-promoting activities of recombinant granulocyte-macrophage colony-stimulating factor (rGM-CSF), recombinant granulocyte colony-stimulating factor (rG-CSF), and recombinant interleukin 3 on primary and secondary colony formation by blast progenitors (leukemic colony-forming units;L-CFU) from patients with acute myeloblastic leukemia (AML) were examined using blast colony assay and compared to colony promotion stimulated by pytohemaggulutinin-stimulated leukocyte-conditioned medium (PHA-LCM). Recombinant GM-CSF stimulated blast colonies in 13 out of 20 cases examined. The magnitude of stimulation by rM-CSFf varied significantly according to the type of AML, but in general was lower than that of PHA-LCM. Blast cells in type M1 did not form any colonies with rGM-CSF, and type M4 blasts formed fairy large numbers of colonies, though slightly less than those stimulated by PHA-LCM. Recombinant G-CSF stimulated blast colonies in only 5 out of 21 cases, 3 of them being type M2. The n
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umber of cases responding to rG-CSF was significantly smaller than that responding to rGM-CSF, and the magnitude of stimulation was minimal. Recombinant G-CSF is not likely to play an essential role in the proliferation of leukemic blasts of most types. Recombinant IL-3 stimulated blast colonies in 12 out of 27 cases examined, however, the magnitude of stimulation by rIL-3 was significantly lower than that by PHA-LCM. No preferential growth of blast colonies was observed according to the AML subtype. Frequency and magnutude of growth stimulation by rIL-3 were not significantly different to those seen by rGM-CSF or RG-CSF. Previous exposure to rGM-CSF, rG-CSF or rIL-3 did not alter the self-renewal capacity, cellular phenotype, and morphology of colony cells, indicating that the direction and the degree of differentiation of L-CFU stimulated by rGM-CSF, rG-CSF or rIL-3 were not different from those stimulated with PHA-LCM. Simulateneous addition of rIL-3 with rGM-CSF or rG-CSF revealed variable results; a simple additive effect in a half of cases studied, and a significantly higher or lower values in the remaining cases. These results may indicate heterogenous responsiveness to CSFs on leukemic blast cells. Less
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Report
(3 results)
Research Products
(10 results)
