| Project/Area Number |
62570580
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General surgery
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| Research Institution | The third department of surgery,Tokyo Women's Medical College |
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Principal Investigator |
TERAOKA Satoshi Associated professor, 第三外科, 教授 (40090659)
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| Co-Investigator(Kenkyū-buntansha) |
KAWAI Taturo Surgical assistant (40186044)
FUCHINOUE Shohei Surgical assistant (10147382)
HONDA Hiroshi Surgical assistant (30147384)
寺岡 慧 東京女子医科大学, 第三外科, 助教授 (20147383)
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| Project Period (FY) |
1988 – 1989
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1988: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1987: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | Liver transplantation / 31P-NMR spectrometer / 31P-NMR / spectroscopy / 肝保存 / 温阻血 |
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| Research Abstract |
Intracellular high energy phosphate of the preserved mouse liver was measured by 31P-MMR spectrometer The measurements were done either under warm or cold ischemic condition. After ischimea, the liver was reperfused with modified Krebs-Henseleit buffer solution and reevaluted.
ATP decreased rapidly and became undetectable within 10 min. under both warm and cold ischemia.
Synthesis of ATP was observed again when reperfusion was started on these ischemically preserved liver. ATP was resynthesised in the liver preserved shorter than 30 min. in case of warm ischemia and 10 hr. in case of cold ischemia. Infusion of the Ca channel blocker(verapamil) in to the portal vein prior to hepatectomy was protective against a decrease in the synthesis of ATP at the time of reperfusion. The ratio of MP/Pi was maintained at high level in the liver which showed good recovery of ATP at the time of reperfusion.
OUR results demonstrated the fact ATP has limited value on judgement of the viability of the preserved organ particulary under non-perfused state. While the MP/Pi ratio may be useful as an indicator available to the same condition.
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