| Budget Amount *help |
¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1988: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1987: ¥900,000 (Direct Cost: ¥900,000)
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| Research Abstract |
Flow cytometric DNA analysis was performed on 68 paraffin-embedded parathyroid tumors from 49 patients with primary hyperparathyroidism (15 cancers-13 primary and 11 locally recurrent or metastatic lesions; 30 adenomas; and 5 hyperplasia-15 glands). Unequivocal evidence of aneuploidy was found in 27% of cancer, 10% of adenoma, and 0% of hyperplasia cases. All 4 patients with aneuploid carcinoma have shown local recurrence or/and distant metastases and have required reexplorations, one of whom died of the disease. Of the 11 patients with non-aneu-ploid carcinoma, 7 patients have no recurrence 2 to 5 years after the initial operation, and 4 showed recurrence. Of the latter, however, 2 are living with normocalcemia 3 and 6 years after reoperation and 2 other are alive with mild hypercalcemia. Thus this study indicates that aneuploid parathroid carcinoma are likely to show more malignant behavior than non-aneuploid ones, although aneuploidy in paraffin materials is not incompatible with a benign histologic diagnosis of parathyroid tumors.
Sixty-three patients operated on for primary hyperparathyroidism had DNA analysis from the excised fresh parathyroid tumors. Three of the tumors were metastatic lesions of parathyroid carcinoma. The remaining 49 primary lesions included 4 histologic carcinoma, 41 adenoma, and 4 hyperplasia (MEN type 1). Unequivocal evidence of aneuploidy was found in 100% of metastatic carcinoma, 75% of histologic carcinoma, and 8% of adenoma, and 50% of hyperplasia cases. Thus parathyroid carcinomas are apt to be aneuploid than adenomas. It is concluded that in patients with nonfamilial primary hyperparthyroidism, flow cytometric DNA analysis of parathyroid tumors from fresh meterials must be a valuable adjunct to the pathological diagnosis of carcinoma.
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