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Nuclear DNA analysis of genetics of cancer and dysplasia complicating ulcerative colitis

Research Project

Project/Area Number 62570593
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Digestive surgery
Research InstitutionUniversity of Tokyo

Principal Investigator

NAGAI Hideo (1988)  First Department of Surgery, Tokyo University, 医学部(病), 助手 (00164385)

杉山 政則 (1987)  東京大学, 医学部(病), 助手 (20192825)

Co-Investigator(Kenkyū-buntansha) SUZUKI Kimitaka  First Department of Surgery, Tokyo University, 医学部第1外科, 医員 (60221321)
MASAKI Tadahiko  First Department of Surgery, Tokyo University, 医学部第1外科, 医員 (30238894)
NAGASHIMA Ikuo  First Department of Surgery, Tokyo University, 医学部第1外科, 助手 (90202423)
SENICHIRO Agawa  First Department of Surgery, Tokyo University, 医学部第1外科, 助手 (00175788)
永井 秀雄  東京大学, 医学部(病), 助手 (00164385)
Project Period (FY) 1987 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1988: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1987: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsMicrospectrophotometry / DNA / Ulcerative colitis / Dysplasia / Aneuploid / Polyploid / Surueillance colonoscopy / 細胞核DNA量 / 癌化
Research Abstract

1)Assessment of optimum thickness of the sections : This study assessed the optimum thickness of the paraffin embedded sections to determine the true DNA content with a cytophofometric technique in 35 colonic samples from 23 patients with ulcerative colitis and 10 non-colitic patients with colorectal cancer. The 4 ,7 ,10 sections were cut from each samples. The cellular DNA values of these sections were compared with smears from cells detached from the same paraffin embedded tissue. The modal DNA values of the four methods were similar. On the contrary, the frequency of the cells with DNA content higher than 6C value (polyploid cells) did not coincide. The frequency of false negative cases which had polyploid cells in smears but hadn't in sections was 8.6, 0, 5.7 in 4, 7, 10 respective. The frequency of false positive cases was 14.3, 20, 25.7, but 6.3, 6.7, 18.8 in the inactive specimens. Therefore 7 section showed higher sensitivity and specificity. We suqqest that micro spectrophotom … More etric DNA content in ulcerative colitis is better analized with 7 section.
2)Application for cancer and dysplasia diagnpsis : The DNA content was measured by microspectrophotometry from 100 specimens in 60 patients with ulcerative colitis including six complicating carcinoma. In dysplastic 23 (77%) of 30 samples showed aneuploidy or polyploidy, whereas in non-dysplastic tissues 50 (94%) of 53 samples were diploid, the difference being statistically significant. Polyploidy was often observed in the non-dysplastic mucosa of the patients complicating cancer or dysplasia. In non-dysplastic patients all sample with inflammation showed diploidy, however 10% of samples without inflammation showed polyploidy. A good correlation was found between the incidence of polyploid cells and the grade of dyspasia. From this study it was assumed that microspectrophotometric measurement of DNA content is useful in the assessment and siagnosis of dysplasia in ulcerative colitis and can be available in the screening of high-risk patients. Less

Report

(3 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] 鈴木公孝 他: 日本大腸肛門病学会雑誌.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 鈴木公孝 他: 日本消化器病学会雑誌.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 正木忠彦 他: 胃と腸. 24. (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 正木忠彦 他: 日本外科学会雑誌. 89. 1315 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kimitaka S.; et al:"Microspectrophotanetric DNA analysis in ulcerative colitis (Report 1) - with special referrence to the assessment of optimum thickness of the sections" The Journal of the Japan society of colo-proctology.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kimitaka S.; et al:"Microspectrophotanetric DNA analysis in ulcerative colitis with special referrence to its application for cancer and dysplasia diaganosis." Japanese Journal of Gastroenterology.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tadahiko M.; et al:"A microspectrophotometric study of colorectal adenomas" Stomach and Intestine. 24. 1989

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tadahiko M.; et al:"A microspectrophotometric study of the nuclear DNA content of coloretal adnoma with reference to its grade of atypia" Journal of Japan Surgical Society. 89. 1315 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 正木忠彦 他: 胃と腸. 24. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 正木忠彦 他: 日本外科学会雑誌. 89. 1315 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 鈴木公孝 他: 日本大腸肛門病学会雑誌.

    • Related Report
      1988 Annual Research Report
  • [Publications] 鈴木公孝 他: 日本消化器病学会雑誌.

    • Related Report
      1988 Annual Research Report
  • [Publications] 阿川千一郎: 胃と腸. 21-9. 987-990 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 武藤徹一郎: 消化器外科. 10(12). 1796-1803 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 小西文雄: Medicina. 24. 208-209 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 正木忠彦: 発表予定(第5回消化器癌DNA研究会).

    • Related Report
      1987 Annual Research Report
  • [Publications] 正木忠彦: 発表予定(第29回日本消化器病学会).

    • Related Report
      1987 Annual Research Report

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Published: 1987-04-01   Modified: 2017-04-05  

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