| Project/Area Number |
62570599
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Digestive surgery
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| Research Institution | Medical Research Institute, Tokyo Medical and Dental University |
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Principal Investigator |
SAKAMOTO Shinobu Med.Res.Inst., Tokyo Med. & Dent. Univ., 難治疾患研究所, 講師 (80107317)
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| Co-Investigator(Kenkyū-buntansha) |
OKAMOTO Ryohei Med.Res.Inst., Tokyo Med. & Dent. Univ., Professor, 難治疾患研究所, 教授 (00013912)
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| Project Period (FY) |
1987 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1988: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1987: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Familial polyposis coli (FPC) / DNA-synthesizing enzyme / Thymidine kinase isozyme / モノクローナル抗体 / 家族性大腸ポリポージス / チミジン・キナーゼ・アイソザイム / DNA合成系酵素 |
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| Research Abstract |
Familial polyposis coli(FPC), a genetic disorder characterized by the development of numerous adenomatous polyps in the colon and rectum, is asociated with a high incidence of coloreetal careinoma; thus this disease reprosents a case of herdtable predisposition to the development of cancer, and is a useful model for the study of biochemical mechanisms underlying carcinogenesis. In terms of thymidine kinase(TK) activity, colon carcinoma tissue was incerased to about 3-fold, whereas FPC polyp samples from 15 patients chowed an average elevation of only 1.8-fold over normal colon tissue. TK isozyme analysis revealed variable patterns of peak A activity, with the cytosolic isozyme being elevated in some cases (8 of 15) and remaining low in others. And serum TK measurements have shown to be of prognostic use in lung cancer, lymphoma, leukemias and colorectal cancer. The sensitivity and specificity of such measurements would be further enhanced if a radioimmunoassay which utilized an antibody specific for the cytosolic isozyme could be developed. Accordingly, we are also presently trying to generate just this monoclonal antibody.
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