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HISTOPATHOLOGICAL CHARACTERISTICS OF THE EARLY STAGE AND ONCOGENOUS EXPRESSION IN PRIMARY HEPATOCELLULAR CARTINOMA

Research Project

Project/Area Number 62570624
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Digestive surgery
Research InstitutionHyogo Medical University

Principal Investigator

OKAMOTO Eizo  Hyogo college of medicine, First department of surgery, professor, 医学部, 教授 (50068425)

Co-Investigator(Kenkyū-buntansha) ORIYAMA Takeshi  Hyogo college of medicine, First department of surgery, assistant, 医学部, 助手 (00185688)
MITSUNOBU Masao  Hyogo college of medicine, First department of surgery, assistant, 医学部, 助手 (70148667)
YAMANAKA Naoki  Hyogo college of medicine, First department of surgery, lecturer, 医学部, 講師 (90131599)
FUJIMOTO Jiro  Hyogo college of medicine, First department of surgery, assistant, 医学部, 助手 (90199373)
TOYOSAKA Akihiro  Hyogo college of medicine, First department of surgery, assistant professor, 医学部, 助教授 (20068498)
能勢 勝義  兵庫医科大学, 医学部, 助手 (30189401)
田中 信孝  兵庫医科大学, 医学部, 講師 (80122323)
Project Period (FY) 1987 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1989: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1988: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1987: ¥900,000 (Direct Cost: ¥900,000)
KeywordsEarly hepatocellular carcinoma / Cytomorphological characteristics / Cytometrical analysis / Immunohistochemistory / DNA ploidy / Onchogenous expression / 免疫組織化学 / フローサイトメトリー / 核DNA解析 / 細小肝癌 / 微小肝癌の形態 / 肝線腫 / 肝硬変 / α-フェトプロテイン
Research Abstract

Early diagnosis - treatment is essential for improving the prognosis of hepatocellular carcinoma (HCC) However, the Pathomorphology of this disease in the early stage has hardly be elucidated.
In the totally 472 resected cases of HCC, 111 cases of small liver cancer(less than 3cm in diameter) containing of 23 cases of minute liver cancer(less than 2 cm in diameter) were investigated by cytomorphological analysis, immunohistochemical study, flow cytometric study on nuclear DNA analysis and expression of cellular oncogenes.
1)In the histology of non-cancerous region not only completed cirrhosis of type B but also a precirrhotic lesion from chronic hepatitis to type B'cirrhosis were observed in a considerable number, especially in large liver cancers.
2)Minute liver cancers showed extracapsular invasion in 60%, intrahepatic metasis in 25%. Considerable advance was observed even when the tumor was less than 2cm in diameter. The HCC with slight or mild cirrhosis revealed the low rates of invas … More ion and metastasis.
3)Small liver cancer consisted primarily of differentiated cells of Ed I and II types.
4)In the distant areas from main tumors, minute daughter masses with no capsule and poorly defined border, consisting smaller, proliferative hepatocellular-cells were observed, and this lesions have been frequently called adenomatous hyperplasia. However, this minute mass showed high N/C ratio in the cytometry, and often had small foci of HCC, though showing negative tumor makers in the immunohistochemistry. Therefore we consider this minute focus to be, indeed, the early lesion of UCC.
5)In the DNA ploidy, small liver cancers showed a significantly higher rate of diploid pattern compared with large tumors.
6)In the oncogenous expression, 5 oncogenes of C-myc, C-Ha-ras, N-ras. lca and C- fos were examined in 17 HCC, and N-ras and C-myc were elevated in nearly all cases. However no oncogenous expression were detected in the minute cancers.
7)The morphology of a early stage of HCC was suggested to be highly well-differentiated cytomorphologic features that show few deviations from normal liver cells. Less

Report

(4 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • 1987 Annual Research Report
  • Research Products

    (34 results)

All Other

All Publications (34 results)

  • [Publications] Okamoto Eizo: "Current status of Hepatic resection in the treatment of Hepatocellular Carcinoma" Neoploms of the liver Editors K.Okuda,K.Gishak. 353-365 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 加藤年啓: "5cm以下小型肝癌切除の予後に関する検討" 兵庫県全外科医会誌. 94. 41-46 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 藤元治朗: "フロ-サイトメトリ-によるパラフィンブロックよりの肝硬変・肝癌の核DNA解析" フロ-サイトメトリ-. 8. 129-131 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 岡本英三: "肝臓癌" 消化器外科. 12. 579-587 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 藤元治朗: "肝細胞癌の核DNA解析" 日本外科学会雑誌. 90. 1568-1570 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Imamura T: "Expression of cellular oncogenes in primary human hepatocellular carcinoma" submitted to cancer.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 光信正夫: "Fibrolamellar hepatomaの臨床病理学的研究" 第87回日本外科学会総会(東京). (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 光信正夫: "肝癌の免疫組織学的検討ー特にCEAを中心としてー" 第24回日本肝臓学会総会. (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 豊坂昭弘: "肝細胞癌の前癌病変及び境界領域病変の臨床病理学的研究ー小肝癌肝切除例における検討ー" 第26回日本癌治療学会総会(新潟). (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 光信正夫: "肝細胞癌の前癌病変及び類似病変の臨床病理学的研究" 第33回日本消化器外科学会総会(東京). (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Okamoto E., Yamanaka N. and Toyosaka A.: "Current status of Hepatic resection in the treatment of Hepatocellular Carcinoma" Neoplasms of the liver Editers. K Okuda, K.G Ishak 353-365, Springer-Verlag, Tokyo, 1987.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Toshihiro Kato, Eizo Okamoto, Akihiro Toyosaka, Naoki Yamanaka, Masao Mitsunobu and Jiro Fujimoto: "Long-term results of small sized hepatocellular carcinoma after hepatectomy" Journal of Hyogo Association of Surgeons, 94, 41-46, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Fujimoto J., Okamoto E., Toyosaka A., Yamanaka N. and Mitsunobu M.: "Flow Cytometric Study on Nuclear DNA analysis of Paraffin-embedded block of Liver cirrhosis and Hepatocellular Carcinoma" Flowcytometry, 8, 129-131, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Okamoto E.: "Hepatocellular Carcinoma" J. of Gastroenterological Surgery, 12, 579-587, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Fujimoto J., Okamoto E., Yamanaka N., Fujiwara S., Mitsunobu M. and Toyosaka A.: "Nuclear DNA analysis of Hepatocellular carcinoma" J. Jap. Surg. Soc., 90, 1568-1570, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Imamura T., Ochiya T., Shiozawa M., Araki M., Mitsunobu M., Okamoto E. and Matsubara K.: "Expression of cellular oncogenes in primary human hepatocellular Carcinoma" Submitted to Cancer.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Mitsunobu M., Okamoto E., Toyosaka A., Yamanaka N. and Fujiwara S.: "A clinicopathological study of Fibrolamellar hepatoma" The 87 Annual Meeting of J. Jap. Surg. Soc. April 3, 1987 (oral presentation).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Mitsunobu M., Okamoto E., Toyosaka A., Yamanaka N. and Fujiwara S.: "Immunohistochemistory of hepatocellular carcinoma -with special reference to CEA-" Acta Hepa. Jap., 29, (supplement) 85, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Toyosaka A., Okamoto E., Mitsunobu M., Yamanaka N. and Fujimoto J.: "A Clinicopathological study of Precancerous lesions and borderline malignancies in hepatocellular Carcinoma" The 26 Annual Meeting of Japan Society for Cancer Therapy Oct. 1, 1988 (presentation).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Mitsunobu M., Okamoto E. and Toyosaka A.: "A clinicopathological study of precancerous lesions and borderline malignancies in hepatocellular carcinoma" The 33 Annual Meeting of Japanese Gastroenterological Surgery Jan. 24, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Okamoto Eizo: "Current status of Hepatic resection in the treatment of Hepatocellular Carcinoma" Neoploms of the liver Editors K.Okuda,K.Gishak. 353-365 (1987)

    • Related Report
      1989 Annual Research Report
  • [Publications] 加藤年啓: "5cm以下小型肝癌切除の予後に関する検討" 兵庫県全外科医会誌. 94. 41-46 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] 藤元治朗: "フロ-サイトメトリ-によるパラフィンブロックよりの肝硬変・肝癌の核DNA解析" フロ-サイトメトリ-. 8. 129-131 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] 岡本英三: "肝臓癌" 消化器外科. 12. 579-587 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 藤元治朗: "肝細胞癌の核DNA解析" 日本外科学会雑誌. 90. 1568-1570 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Imamura T: "Expression of cellular oncogenes in primary human hepatocellular carcinoma" submitted to cancer.

    • Related Report
      1989 Annual Research Report
  • [Publications] 光信正夫: "Fibrolamellar hepatomaの臨床病理学的研究" 第87回日本外科学会総会(東京). (1987)

    • Related Report
      1989 Annual Research Report
  • [Publications] 光信正夫: "肝癌の免疫組織学的検討-特にCEAを中心として-" 第24回日本肝臓学会総会. (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] 豊坂昭弘: "肝細胞癌の前癌病変及び境界領域病変の臨床病理学的研究-小肝癌肝切除例における検討-" 第26回日本癌治療学会総会(新潟). (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] 光信正夫: "肝細胞癌の前癌病変及び類似病変の臨床病理学的研究" 第33回日本消化器外科学会総会(東京). (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 岡本英三: 第24回日本肝臓学会総会 1988.7.

    • Related Report
      1988 Annual Research Report
  • [Publications] 岡本英三: 第24回日本肝癌研究会 1988.7.

    • Related Report
      1988 Annual Research Report
  • [Publications] 岡本英三: 兵庫県全外科医会誌. 94. 41-46 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 岡本英三: フローサイトメトリー. 8. 129-131 (1988)

    • Related Report
      1988 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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