Grant-in-Aid for General Scientific Research (C)
|Allocation Type||Single-year Grants |
|Research Institution||Hyogo College of Medicine |
OKAMOTO Eizo Hyogo college of medicine, First department of surgery, professor, 医学部, 教授 (50068425)
ORIYAMA Takeshi Hyogo college of medicine, First department of surgery, assistant, 医学部, 助手 (00185688)
MITSUNOBU Masao Hyogo college of medicine, First department of surgery, assistant, 医学部, 助手 (70148667)
YAMANAKA Naoki Hyogo college of medicine, First department of surgery, lecturer, 医学部, 講師 (90131599)
FUJIMOTO Jiro Hyogo college of medicine, First department of surgery, assistant, 医学部, 助手 (90199373)
TOYOSAKA Akihiro Hyogo college of medicine, First department of surgery, assistant professor, 医学部, 助教授 (20068498)
能勢 勝義 兵庫医科大学, 医学部, 助手 (30189401)
田中 信孝 兵庫医科大学, 医学部, 講師 (80122323)
|Project Period (FY)
1987 – 1989
Completed (Fiscal Year 1989)
|Budget Amount *help
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1989: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1988: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1987: ¥900,000 (Direct Cost: ¥900,000)
|Keywords||Early hepatocellular carcinoma / Cytomorphological characteristics / Cytometrical analysis / Immunohistochemistory / DNA ploidy / Onchogenous expression / 免疫組織化学 / フローサイトメトリー / 核DNA解析 / 細小肝癌 / 微小肝癌の形態 / 肝線腫 / 肝硬変 / α-フェトプロテイン|
Early diagnosis - treatment is essential for improving the prognosis of hepatocellular carcinoma (HCC) However, the Pathomorphology of this disease in the early stage has hardly be elucidated.
In the totally 472 resected cases of HCC, 111 cases of small liver cancer(less than 3cm in diameter) containing of 23 cases of minute liver cancer(less than 2 cm in diameter) were investigated by cytomorphological analysis, immunohistochemical study, flow cytometric study on nuclear DNA analysis and expression of cellular oncogenes.
1)In the histology of non-cancerous region not only completed cirrhosis of type B but also a precirrhotic lesion from chronic hepatitis to type B'cirrhosis were observed in a considerable number, especially in large liver cancers.
2)Minute liver cancers showed extracapsular invasion in 60%, intrahepatic metasis in 25%. Considerable advance was observed even when the tumor was less than 2cm in diameter. The HCC with slight or mild cirrhosis revealed the low rates of invas
ion and metastasis.
3)Small liver cancer consisted primarily of differentiated cells of Ed I and II types.
4)In the distant areas from main tumors, minute daughter masses with no capsule and poorly defined border, consisting smaller, proliferative hepatocellular-cells were observed, and this lesions have been frequently called adenomatous hyperplasia. However, this minute mass showed high N/C ratio in the cytometry, and often had small foci of HCC, though showing negative tumor makers in the immunohistochemistry. Therefore we consider this minute focus to be, indeed, the early lesion of UCC.
5)In the DNA ploidy, small liver cancers showed a significantly higher rate of diploid pattern compared with large tumors.
6)In the oncogenous expression, 5 oncogenes of C-myc, C-Ha-ras, N-ras. lca and C- fos were examined in 17 HCC, and N-ras and C-myc were elevated in nearly all cases. However no oncogenous expression were detected in the minute cancers.
7)The morphology of a early stage of HCC was suggested to be highly well-differentiated cytomorphologic features that show few deviations from normal liver cells. Less