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Neural differential antigen and oncogene in brain tumor

Research Project

Project/Area Number 62570653
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Cerebral neurosurgery
Research InstitutionNagoya University

Principal Investigator

JUN YOSHIDA  Dept. of Neurosurgery, Nagoya University School of Medicine, 医学部, 助手 (40158449)

Co-Investigator(Kenkyū-buntansha) ITARU INOUE  Dept. of Endo crinology and Metabolism, Research Institute of Environmental Medi, 環境医学研究所, 助手 (80184737)
HISAO SEO  Dept. of Endo crinology and Metabolism, Research Institute of Environmental Medi, 環境医学研究所, 助教授 (40135380)
Project Period (FY) 1987 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1988: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1987: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsGFAP / NSE / fibronectin / monoclonal antibody / oncogene / glioma CDNA / cDNA / 神経分化抗原 / 脳腫瘍 / グリオーマ関連抗原 / 発癌遺伝子
Research Abstract

A variety of human glioma tissues and human glioma cell lines was solubilized with 1% NP-40 and analyzed by Western blotting method. Four neural differential antigens; glial fibrillary acidic protein, neuron specific enolase, fibronectibn, and a glioma associated antigen defined by our G-22 monoclonal antigen, were demonstrated as a single band of 40-50 Kd, 50 Kd, 230 Kd, and 67 Kd respectively on SDSPASE. Quantitative determination of their antigens expressed in glioma cells was measured by the method of dot blot, Elisa, or RIA and geterogeneity concerning the level of neural differential antigen was revealed and it was also noted that the expression of GFAP was reversely correlated with that of fibronectin and G-22 antigen. On the other hand it has been reported that a variety of oncogene was expressed on human neoplasm include glioma and they are deeply related with their oncogenesis, growth, and differentiation. We confirmed the expression of N-ras, N-myc, C-myc, and C-fos oncogen in human glioma cell lines by northern blot analysis and also noted that the expression of C-fos gene was increased by the stimulation of a differentiation factor, nicotinamide. However overexpression of these oncogene or any positive relation to the expression of neural differentiation antigens were not demonstrated in the present study. We have already succeeded to clone the cDNA of human GFAP and now we are investigating the relation between oncogene and neural differentiation antigens at DNA level.

Report

(3 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Yoshida,J,;Wakabayashi,T,;Kito,A,;Kageyama,N,;Murata,Y,;Seo,H,;Kojima,N,;Yagi,K;: Prog exp Tumor Res. 30. 255-267 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Wakabayashi,T,;Yoshida,J,;Seo,H,;Kato,K,;Murata,Y,;Matsui,N,;Kageyama,N.: J.Neurosurg. 68. 449-455 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yoshida,J,;Yamamoto,R,;Wakabayashi,T,;Nagata,M,;Seo,H.: J Neuro-oncology.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 吉田純: 細胞. 19. 209-213 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 井上達、吉田純: 細胞. 20. 480-484 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 井上達,妹尾久雄,古橋円,神戸福司,村田善晴,松井信夫,吉田純: 名古屋大学環境医学研究所年報. 39. 207-211 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yoshida J; Wakabayashi T; Kito A; Kageyama N; Murata Y; Seo H; Kojima N; Yagi K: "Clinical application of monoclonal antibody against glioma-associated antigen." Prog exp Tumor Res.30. 255-267 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Wakabayashi T; Yoshida J; Seo H; Kato K; Murata Y; Matsui N; Kageyama N: "Characterization of neusoectodermal antigen by a monoclonal antibody and its application in CSF diagnosis of human glioma" J. Neun surgery. 68. 449-455 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yoshida J; Yamamoto R; Wakabayashi T; Nagata M; Seo H: "Radioimmuno assay of glioma-associated antigen in cerebrospinal fluid and its usefulness for the diagnosis and monitoring of human glioma" J. Neun oncology. in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 井上達,妹尾久雄,丹羽靖,神部福司,村田善晴,吉田純,松井信夫: 名古屋大学環境医学研究所年報. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Wakabayashi T.;Yoshida J.;Seo,H.;Kato,K.;Murata,Y.;Matsui,N.;Kageyama,N.: J.Neurosurg. 68. 449-455 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Yoshida,J.;Yamamoto,R.;Wakabayashi,T.;Nagata,M.;Seo,H.: J.Neuro-oncology.

    • Related Report
      1988 Annual Research Report
  • [Publications] 吉田純: Annual Review神経1988. 180-187 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 井上達,吉田純: 細胞. 20. 480-484 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 井上達,妹尾久雄,古橋円,神戸福司,村田善晴,松井信夫,吉田純: 名古屋大学環境医学研究所年報. 39. 207-211 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 吉田純: 細胞. 19. 209-213 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Yoshida, J.: Prog. Exp. Tumor Res.30. 44-56 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 吉田純: "Annual Revies 神経 1988" 中外医薬社, 363 (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] 吉田純: "図説臨床「癌」シリーズ No. 15 脳腫瘍" メジカルビュー, 176 (1987)

    • Related Report
      1987 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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