| Budget Amount *help |
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1988: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1987: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Research Abstract |
A variety of human glioma tissues and human glioma cell lines was solubilized with 1% NP-40 and analyzed by Western blotting method. Four neural differential antigens; glial fibrillary acidic protein, neuron specific enolase, fibronectibn, and a glioma associated antigen defined by our G-22 monoclonal antigen, were demonstrated as a single band of 40-50 Kd, 50 Kd, 230 Kd, and 67 Kd respectively on SDSPASE. Quantitative determination of their antigens expressed in glioma cells was measured by the method of dot blot, Elisa, or RIA and geterogeneity concerning the level of neural differential antigen was revealed and it was also noted that the expression of GFAP was reversely correlated with that of fibronectin and G-22 antigen. On the other hand it has been reported that a variety of oncogene was expressed on human neoplasm include glioma and they are deeply related with their oncogenesis, growth, and differentiation. We confirmed the expression of N-ras, N-myc, C-myc, and C-fos oncogen in human glioma cell lines by northern blot analysis and also noted that the expression of C-fos gene was increased by the stimulation of a differentiation factor, nicotinamide. However overexpression of these oncogene or any positive relation to the expression of neural differentiation antigens were not demonstrated in the present study. We have already succeeded to clone the cDNA of human GFAP and now we are investigating the relation between oncogene and neural differentiation antigens at DNA level.
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