| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1988: ¥100,000 (Direct Cost: ¥100,000)
Fiscal Year 1987: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Research Abstract |
Malignant brain tumor cell lines resistant to colchicine at concentrations of 5, 10 and 30 ng/ml were newly established from rat C6 glioma, human KY cell and KC cell lines. Among them, the cell lines which also resist to both adriamycine and vincristine were selected. From these selected cell lines we have established the new cell lines C6-Ch^R-5-8-6, KY-CH^R-8-9-4 and KC-Ch^R-1-2-5, which showed a high resistance about 5-10 times to colchicine. We found that these colchicine-resistant cell lines also had a resistance to adriamicine, vinblastine vincristine, actinomycin D and procarbazine, but not to ACNU and Cis-DDP as examined by the plaque formation assay. While, retinolacetate and synthetized isoprenoid (SDB-ethylenediamine, PMB-decaprenylamine), which are derivatives of vitamine A suppressed a gain of resistance to colchicine and adriamycine of these cell lines in the following generations. We found that the KC-Ch^R-1-2-5 cell had only a half intracellular concentration of adriamycine, but showed no change in morphologg, protein synthesis and phosphorylation of proteins.
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