| Project/Area Number |
62570669
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Cerebral neurosurgery
|
|---|
| Research Institution | Teikyo University School of Medicine |
|---|
Principal Investigator |
TSUJITA Yoshihiko Assistant Professor, 医学部, 講師 (50163803)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MIZUNO Shigeki Assistant, 医学部, 助手 (60200010)
HOJO Shuntaro Associate Professor, 医学部, 助教授 (70133072)
|
|---|
| Project Period (FY) |
1987 – 1988
|
| Project Status |
Completed (Fiscal Year 1988)
|
| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1988: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1987: ¥1,500,000 (Direct Cost: ¥1,500,000)
|
|---|
| Keywords | グリオーマ / トランスフェリン / トランスフェリン受容体 / フローサイトメトリー / 細胞内カルシウムイオン / transferrin / glioma / glial fibrillary acidic protein / vimentinSDS-PAGE / 細胞周期 / 細胞内カルシウムイオン濃度 |
|---|
| Research Abstract |
As a part of study of neuroglial cell function we studied an evidence of transferrin receptor molecules in cultured human glioma cells.
SDS-polyacrylamide gel electrophoresis and fluorography of ^<35>S methionine biolabeled glioma cell lystates immunoprecipitated with anti-traniserrin receptor antibody showed bands of Mr=94,000 in the reducing condition. When the cells were stained with FITC-conjugated anti-transferrin receptor antibody flow cytometric histogram revealed the abundant fluorescence due to cell surface transferrin receptor molecules. Two dimensional flow cytometric analysis demonstrated that cell surface transferrin receptor positive cells existed in each phase of the cell cycle.
Furthormore using quin2 loading method, we measured the intracellular concentration of free-Ca^<2+> ion level in glioma cells. The result indicated that transferrin induced the elevation (30 - 50 %) of free-Ca^<2+> concentraion for observed time intervals (5 min).
These results suggest that glioma cells might be regulated for cell function and metabolism fhrough membrane associated receptors by transferrin.
|
