Analysis of Collagen gene from the Patient with Osteogenesis Imperfecta

Research Project

Project/Area Number	62570686
Research Category	Grant-in-Aid for General Scientific Research (C)
Allocation Type	Single-year Grants
Research Field	Orthopaedic surgery
Research Institution	Medical Collage of Oita
Principal Investigator	NAKAMURA Michitoshi Medical Collage of Oita, Assistant Professor, 医学部, 講師 (00145385)
Project Period (FY)	1987 – 1988
Project Status	Completed (Fiscal Year 1988)
Budget Amount *help	¥2,300,000 (Direct Cost: ¥2,300,000) Fiscal Year 1988: ¥500,000 (Direct Cost: ¥500,000) Fiscal Year 1987: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywords	Osteogenesis Imperfecta(OI) / 2(I) collagen gene / cDNA / molecular cloning / cDNA / α2(I)コラーゲン遺伝子 / 制限酵素多型(RFLP) / 日本人 / コラーゲン遺伝子 / RFPM(Restriction Fragments Poly Morphism)
Research Abstract	Collagen gene,especially 2(i), was studied for the concerns with Osteogenesis Imperfecta. Reseach plan was consisted with two major direction. 1. Direct analysis of the cDNA from the patient with oI. 2. Gene screening by the PFLP. For carrying out these plans, i asked Dr.Prockod(Jefferson University, Philadel-phia USA) for 2(i)collagen cDNA gene, courteouly Dr. Kuivaniemi who was a collaborator with Dr. Prockop sent me the full length cDNA. 1. Apatient with oI was treated with surgical procedure and when skin was resected. This sample was cutted into small blockes and cultured. But this sample was contaminated with bacteria, so I could not get the mPNA from the sample. 2. I prepaired 173 persons DNA samples from blood. And i tried non-radioactive detection system, because I want to use this Southrn anlysis as the clinical tests. Finally these systems worked well. First report will appear in Sep. 1989 in the meeting of the Japan Orthopedic Ass. Concerning to the EcoRI fragments in the Japanese.