• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of Collagen gene from the Patient with Osteogenesis Imperfecta

Research Project

Project/Area Number 62570686
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Orthopaedic surgery
Research InstitutionMedical Collage of Oita

Principal Investigator

NAKAMURA Michitoshi  Medical Collage of Oita, Assistant Professor, 医学部, 講師 (00145385)

Project Period (FY) 1987 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1988: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1987: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsOsteogenesis Imperfecta(OI) / 2(I) collagen gene / cDNA / molecular cloning / cDNA / α2(I)コラーゲン遺伝子 / 制限酵素多型(RFLP) / 日本人 / コラーゲン遺伝子 / RFPM(Restriction Fragments Poly Morphism)
Research Abstract

Collagen gene,especially 2(i), was studied for the concerns with Osteogenesis Imperfecta. Reseach plan was consisted with two major direction.
1. Direct analysis of the cDNA from the patient with oI.
2. Gene screening by the PFLP. For carrying out these plans, i asked Dr.Prockod(Jefferson University, Philadel-phia USA) for 2(i)collagen cDNA gene, courteouly Dr. Kuivaniemi who was a collaborator with Dr. Prockop sent me the full length cDNA.
1. Apatient with oI was treated with surgical procedure and when skin was resected. This sample was cutted into small blockes and cultured. But this sample was contaminated with bacteria, so I could not get the mPNA from the sample.
2. I prepaired 173 persons DNA samples from blood. And i tried non-radioactive detection system, because I want to use this Southrn anlysis as the clinical tests. Finally these systems worked well. First report will appear in Sep. 1989 in the meeting of the Japan Orthopedic Ass. Concerning to the EcoRI fragments in the Japanese.

Report

(3 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] 中村雅彦,中村道利,真角昭吾: 日本整形外科学会雑誌(抄録特集号). 63. (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Applied Masahiko NAKAMURA; Michitoshi NAKAMURA; Syogo MASUMI: "RFLPs in Japanese collagen gene." J. Jpn. Ortho. Ass.63. (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 中村雅彦、中村道利、真角昭吾: 日本整形外科学会雑誌. 63. (1989)

    • Related Report
      1988 Annual Research Report

URL: 

Published: 1987-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi