STADIES OF THE INTERFERON RECEPTORS AND ANTITUMOR ACTIVITY

Research Project

Project/Area Number	62570727
Research Category	Grant-in-Aid for General Scientific Research (C)
Allocation Type	Single-year Grants
Research Field	Urology
Research Institution	Tokushima University, School of Medicine, Department of Urology
Principal Investigator	SUSUMU KAGAWA Tokushima University, School of Medicine, Dept. of Urology, Professor, 医学部泌尿器科, 教授 (40035738)
Co-Investigator(Kenkyū-buntansha)	YOSHITERU SUMIYOSHI Tokushima University, School of Medicine, Dept. of Urology, Assistant., 医学部泌尿器科, 助手 (80187814)
Project Period (FY)	1987 – 1988
Project Status	Completed (Fiscal Year 1988)
Budget Amount *help	¥800,000 (Direct Cost: ¥800,000) Fiscal Year 1988: ¥300,000 (Direct Cost: ¥300,000) Fiscal Year 1987: ¥500,000 (Direct Cost: ¥500,000)
Keywords	Interferon / Imterferon receptor / インターフェロンレセプター / 併用効果 / インターフエロンα / インターフエロンγ / インターフエロンレセプター / インターフェロン / レセプター / 腎細胞癌培養株 / 膀胱腫瘍培養株 / I-125ラベルインターフェロン
Research Abstract	Correlation between antiproliferative and binding activities of Interferon(IFN)- and IFN- to various human cell lines was examined. The results were as follows. 1) Human renal cell carcinoma KPK-1 cells were sensitive to IFN- , whereas human bladder carcinoma J-82 cells showed no sensitivity in vitro. In these two cell lines, I-125 IFN- binding assay revealed that the difference in IFN- sensitivity was related to the number of the IFN- receptors per cell. 2) BUT some of cell lines showed low sensitivity to FIN, whereas showed having many receptors. Thease results suggest that the cell having a few receptors dose not show sensitivity to IFN. 3) By treatment of TNF, the number of receptor to IFN increased in some cell lines, which is suggest the usefulness for combination therapy of TNF and IFN. 4) By combination of IFN- and IFN- , 3 cell lines of 7 cell lines showed synergistic effect.