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Role of calmoduline binding protein in the mechanism of prolactin secretion

Research Project

Project/Area Number 62570754
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Obstetrics and gynecology
Research InstitutionOsaka University. Department of Obstetrics & Gynecololy

Principal Investigator

KENJI Hirota  Osaka University Medical School. Research fellow, 医学部, 助手 (00189888)

Co-Investigator(Kenkyū-buntansha) KADOWAKI Kozo  Osaka University Medical School. Research fellow, 医学部, 助手 (60194875)
MIYAKE Akira  Osaka University Medical School. Assistant Professor, 医学部, 講師 (90093468)
Project Period (FY) 1987 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1988: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1987: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsProlactin / TRH / GTP binding protein / Calmodulin / IAP / 下垂体細胞 / アラキドン酸 / カルスペクチン / phospholipase A_2 / 下垂体前葉 / 視床下部 / カルシウム
Research Abstract

It is well known that prolactin is released from pituitary gland by TRH and inhibited by dopamine. We tried to clarify the role of calcium and calmodulin in prolactin release by TRH, and the role of guanosine trisphosphate (GTP) binding protein in inhibitory mechanism of PRL by dopamine. Pituitary cells and hypothalamic cells were used for the experiment. Maitotoxin induced Ca2+ influx, arachidonate and prolactin release. These results showed Ca2+ influx induce prolaction release. Prolactin secretion was suppressed by W7, which is an antagonist of calmodulin. Phospholipase A2, activated by Ca2+, inducedarachidonate and prolactin. As a conclusion, we showed that Ca2+ influx stimulates prolactin secretion through calmodulin and phospholipase A2. On the other hand, prolactin secretion is inhibited by dopamine. we tried to clarified whether the islet-activating protein (IAP) sensitive guano sine trisphosphate (GTP) protein is involved in TRH or dopamine receptor. Although IAP did not stimulate prolactin release nor inhibited prolactin release stimulated TRH or phospholipase A2 or arachidonate. IAP in activated dopamine action, which suppressed prolactin secretion stimulated by TRH. These results show that dopamine suppressed prolactin secretion through IAP sensitive GTP protein.

Report

(3 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Final Research Report Summary
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] 広田憲二: 日本内分泌学会雑誌. 63. 515 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 池上博雅: 日本産婦人科学会雑誌. 39. 1371 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 池上博雅: 日本内分泌学会雑誌. 63. 407 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Masahide Ohmichi: Neuroendocrinology.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kozo Kakowaki: Neuroendocrinology.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kenji Hirota: 8th International Congress Endocrinology. 184 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kozo Kadowaki: 8th International Congress Endocrinology. 205

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Masahide Ohmichi: "Involvement of extracellular calcium and arachidonate in [_3H]dopamine release from rat tubelar neurons" Neuroendocrinology.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kozo Kadowaki: "Influence of d-cAMP and forskoline on dopamine uptake in the rat tuberoinfundibular neurons" Neuroendocrinology.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kenji Sobue: "Cytosynalin: A Mr. 35,000 cytoskelton-releasing and calmodulin-binding protein" Pro. Natl. Acad. Sci. USA. 84. 1916-1920 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kenji Hirota: "Roles of lipoxyganase and epoxygenase metabolites of arachidonic acid in release of prolactin from rat pituitary cells" Abstracts of International Congress Endocrinology. 184 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kozo Kadowaki: "Regulation of [_3H]-dopamine accumulation in the medium eminence:Effects of W-7, d-cAMP and bromocriptine" Abstracts of 8th International Congress of Endocrinology. 205 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Mitsu Iwashita: J.mol.Endocrinology. 1. 187-196 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Akira Miyake: Journal of Endocrinological Investigation. 11(5). 383-384 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Masahide Ohmichi: Neuroendocrinology.

    • Related Report
      1988 Annual Research Report
  • [Publications] Kozo Kadowaki: Neuroendocrinology.

    • Related Report
      1988 Annual Research Report
  • [Publications] Kenji Hirota: 8th International Congress Endocrinology. 184 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Kozo Kadowaki: 8th International longress Endocrinology. 205 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 池上博雅: 日本産婦人科学会雑誌. 39. 1371- (1987)

    • Related Report
      1987 Final Research Report Summary
  • [Publications] Kenji Sobue: Pro. Natl. Acad. Sci USA. 84. 1916-1920 (1987)

    • Related Report
      1987 Final Research Report Summary
  • [Publications] 広田憲二: 日本内分泌学会雑誌. 63. 515- (1987)

    • Related Report
      1987 Final Research Report Summary
  • [Publications] Kenji Hirota: Cancer Research. 47. 3742-3746 (1987)

    • Related Report
      1987 Final Research Report Summary
  • [Publications] 池上博雅: 日本内分泌学会雑誌. 63. 407- (1987)

    • Related Report
      1987 Final Research Report Summary
  • [Publications] A. Miyake: Neuroendocrinology. 45. 191-196 (1987)

    • Related Report
      1987 Final Research Report Summary

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Published: 1987-04-01   Modified: 2016-04-21  

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