| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1988: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1987: ¥1,600,000 (Direct Cost: ¥1,600,000)
|
|---|
| Research Abstract |
The present study was conducted to demonstrate in endometriosis peritoneal fluid (PF) an ovum capture inhibitor (OCI) as a causative factor for endometriosis-associated infertility and, if any, to investigate its action mechanism. A mouse oocytecumulus complex was repeatedly applied on a golden hamster oviductal fimbria immersed in endometriosis or non-endometriosis PF. The period from the initiation of the experiment until the termination of fimbrial activity of ovum capture, ovum capture didappearance time (OCDT), was significantly reduced with native, cell-free and macromolecular fractions of endometriosis PF. A prolongation in OCDT was noted with PF specimens taken from endometriosis women under treatment with Danazol, an agent known to increase fecundity in endometriosis. Scanning electron microscopy disclosed membranous deposits (OCI-related membrane) on the fimvria exposed to endometriosis PF, by which ciliated fimbrial epithelial cells were completely concealed. A retrograde tubal flush for the oviduct exposed to endometriosis PF first ballooned and then removed the OCI-related membrane. Flushed oviducts resumed fimbrial activity of ovum capture. Laparoscopy, when performed on endometriosis women whose peritoneal cavity was filled with saline, demonstrated a balloon formation of possible OCI-related membranes on the fimbria under chromotubation. The OCI, reducing fimbrial activity of ovum capture, is abundantly present in endometriosis PF. It is a water-soluble macromolecule. Danazol treatment mught decrease OCI acgtivity. The OCI reduces fimbrial activity of ovum capture through preventing the cumulus-fimbrial interaction by the OCI-related membrane deposited on the oviductal fimbria.
|
