| Project/Area Number |
62570798
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Ophthalmology
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| Research Institution | Aichi Medical University |
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Principal Investigator |
OGINO Nobuchika Associate Professor of Ophthalmology, Aichi Medical University., 医学部, 助教授 (50115812)
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| Co-Investigator(Kenkyū-buntansha) |
NEGI Akira Assistant Professor of Ophthalmology, Kyoto University Faculty of Medicine., 医学部, 講師 (00189359)
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| Project Period (FY) |
1987 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1988: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1987: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Human retinal pigment epithelialcell / Chick retinal pigment epithelial cell Leukotriene B_4 / Prostaglandin D_4 / Chemotaxis / プロスタグランジンD_2 / ロイコトリエンB_4 / 網膜色素上皮細胞 / 網膜前後増殖組織由来細胞 / 遊走能 / ロイコトリエン / プロスタグランジノ / 細胞骨格 |
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| Research Abstract |
Retinal pigment epithelial cell is the most important cellular component in proliferative vitreoretinopathy, and the understanding of its migration mechanism is essential to prevent blindness caused by proliferative vitreoretinopathy. We found that N-formylmethionyl-lecuyl-phenylalanine, a strong chemoattractant of leukocytes, was also chemoattractant for cultured chick retinal pigment epithelial cells, while its concentration needed for the migration was higher than that for leukocyte. Melatonin, of which content is high in the retina but its physiologic function is not yet known, was demonstrated to be a specific methoxyindole derivative showing chemotactic activity on the retinal pigment epithelial cells. melatonin was a more effective chemoattractant than n-formylmethionyl-leucyl-phenylalanine. Arachidonic acid derivatives leukotriene B_4 and 5-hydroxytetraenoic acid, both are well known lekocyte chemoattractants, showed a chemotactic activities on the cultured chick and human retinal pigment epithelial cells. Another arachidonic acid derivative prostaglandin D_2 which is the retina specific prostaglandin showed chemokinetic activity on these cells. Cell migratory activities of arachidonic acid derivatives exhibited also on the cells cultured from surgically removed epiretinal and subretinal proliferative vitreoretinopathy tissues. These results suggested a participation of melatonin, leukotriene B_4 and prostaglandin D_2 in migration of retinal pigment epithelial cells in proliferative vitreoretinopathy.
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