| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1988: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1987: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Research Abstract |
To elucidate the biological property of periodontal pocket epithelium, immunohistochemistry, electron microscopy and tracer methods were employed. Experimental pocket was produced by insertion of elastic gums between the first and second molar of the rat maxilla. In specimens of immunohistochemistry using anti-keratin antibody, pocket epithelium showed positive staining, while normal junctional epithelium revealed negative. Outer basal lamina of both pocket and junctional epithelia showed positive reaction to anti-laminin antibody. In electron microscopic observation the pocket epithelium was polygonal in shape, and had a large nucleus located in the center of the cytoplasm. Poor-developed organelle, few rough endoplasmic reticulum and Golgi apparatus but a small amount of tonofilaments, was detected. Initial changes of periodontal pocket formation could be found, i.e., as a destruction of cell-to cell connection but not an exfoliation of epithelial cells from the tooth surface. It could be detected that tracers passed through intercellular spaces of the pocket epithelium into periodontal pocket in the present stuey using peroxidase and lanthanum. Namely, the pocket epithelium was provided structures allowing to pass some materiasl through tese intercellular spaces. Abundant capillaries were observed in the connective tissues just below the epithelium. On the contrary, few capillaries could be detected in the connective tissue corresponding to the long junctional epithelium. From these results, it is suggested that ; (1) pocket epithelium may be poorly differentiated keratinizing cell, (2) pocket epithelium has structures to allow passing some materials through their intercellular spaces, consequently, pocket epithelium is provided a protective function by cervical fluids in similar manner of normal junctional epithelium, (3) to examine the presence of laminin at the tooth side is effective to determine junctional epithelium or pocket epithelium.
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